Vitagenic Effect of Specific Bioactive Fractions of Rhodiola with Trachurus sp. Extract Against Oxidative Stress-Induced Aging in Human Amnion Derived Epithelial Cell Line: In View of a Novel Senolytic

Title:Vitagenic Effect of Specific Bioactive Fractions of Rhodiola with Trachurus sp. Extract Against Oxidative Stress-Induced Aging in Human Amnion Derived Epithelial Cell Line: In View of a Novel Senolytic

Volume: 14 Issue: 2

Author(s): Francesco Marotta, Sathya Priya Thandavan, Surajit Pathak*, Sushmitha Sriramulu, Ganesan Jothimani, Dharanivasan Gunasekaran, Devaprasad Markandeyan and Antara Banerjee

Affiliation:

• Department of Medical Biotechnology, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education (CARE), Chettinad Hospital and Research Institute (CHRI), Kelambakkam, Chennai 603 103,India

Keywords: Rhodiola, specific bioactive fractions, specific fish lipoprotein extract, anti-oxidant, aging, chemometric, oxidative stress, senolytics.

Abstract:

Background: Rhodiola rosea is a herb that has been used in traditional medicine for several years, and LF is a class of lipoproteins derived from the fish Trachurus sp. (LF-T), which exhibits known anti-inflammatory activity.

Objective: Investigating the anti-aging effect of Rhodiola specific bioactive fractions cluster in combination with LF-T (R-L compound) in H₂O₂ mediated oxidative stress-induced human amnion derived epithelial cell line - FL cells as normal human cell line.

Methods: FL cells were treated with H₂O₂ to induce cellular aging, followed by treatment of the RL compound to study its anti-aging characteristics. Based on the proliferation rate, 0.05% and 0.1% concentration of R-L compound was determined using MTT assay. Anti-aging and anti-oxidant assays, ABTS, DPPH, Hyaluronidase activity Nitric Oxide, Lipid Peroxidase, and Superoxide Dismutase were performed. qPCR for anti-aging genes and matrix metalloproteinase genes were analyzed.

Results: FL cells treated with R-L compound exhibited increased proliferation rate and free-radical reduction. Decreased Hyaluronidase enzyme activity and regulation of genes such as SIRT1, KLOTHO, SERPINA 6, MMP 9, and MMP 2 expression depicted the anti-aging role of the R-L compound. Chemometric profiling of the R-L compound revealed that aromatic compounds and unsaturated fatty acids along with their derivatives, were present predominantly, which might have attributed to the potent oxidative stress impeded aging activity.

Conclusion: Specific Bioactive Fractions of Rhodiola in combination with LF-T obtained from Trachurus sp. involve in the regulation of aging genes and might be a novel approach to prevent the cells from oxidative stress damage and also it might avert the aging of cells.

Cite this article as:

Marotta Francesco , Thandavan Priya Sathya , Pathak Surajit *, Sriramulu Sushmitha, Jothimani Ganesan, Gunasekaran Dharanivasan, Markandeyan Devaprasad and Banerjee Antara , Vitagenic Effect of Specific Bioactive Fractions of Rhodiola with Trachurus sp. Extract Against Oxidative Stress-Induced Aging in Human Amnion Derived Epithelial Cell Line: In View of a Novel Senolytic, Current Aging Science 2021; 14 (2) . https://dx.doi.org/10.2174/1874609814666210114094030