评估可能的处理时间对人类血液样本整体N -糖基化的影响

Title:Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples

Volume: 20 Issue: 10

关键词: 血液取样，储存温度，储存时间，分析前处理，N-glycome，毛细管电泳。

摘要: The utilization of N-glycan profiling recently gained high importance in fundamental biomedical and applied clinical research. However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies. In this paper, the effect of human blood sample handling was studied prior to N-glycosylation profiling by capillary electrophoresis, coupled with high sensitivity fluorescence detection. Special attention was paid to the preservation of sialylated structures because of their important clinical - biological relevance. Our results suggested that it is adequate to refrigerate and store the collected total blood samples prior to analysis to obtain unbiased results. Furthermore, we report on the good practice of serum sample handling in order to prevent decomposition of the sialylated structures. Our findings may promote procedure standardization and easier clinical translation of diagnostic N-glycosylation profiling in molecular medicinal applications.

Cite this article as:

Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples, Current Molecular Medicine 2020; 20 (10) . https://dx.doi.org/10.2174/1566524020666201230094722