Proteome Analysis of CD4⁺ T Cells Reveals Differentially Expressed Proteins in Infertile Polycystic Ovary Syndrome Patients

Title:Proteome Analysis of CD4⁺ T Cells Reveals Differentially Expressed Proteins in Infertile Polycystic Ovary Syndrome Patients

Volume: 21 Issue: 11

Author(s): Fatemeh Nasri, Maryam Zare, Mehrnoosh Doroudchi and Behrouz Gharesi-Fard*

Affiliation:

• Department of Immunology, Shiraz University of Medical Sciences, Shiraz,Iran

Keywords: CD4-Positive T-lymphocytes, cellular metabolism, glycolysis, polycystic ovary syndrome, proteomics, PEBP1 protein, PSME1 protein, Triose-phosphate isomerase 1 protein.

Abstract:

Background: Polycystic ovary syndrome (PCOS) is the most frequent endocrine disorder affecting 6–7% of premenopausal women. Recent studies revealed that the immune system, especially CD4+ T helper cells are important in the context PCOS. Proteome analysis of CD4+ T lymphocytes can provide valuable information regarding the biology of these cells in the context of PCOS.

Objective: To investigate immune dysregulation in CD4+ T lymphocytes at the protein level in the context of PCOS using two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS).

Methods: In the present study, we applied two-dimensional gel electrophoresis / mass spectrometry to identify proteins differentially expressed by peripheral blood CD4+ T cells in ten PCOS women compared with ten healthy women. Western blot technique was used to confirm the identified proteins.

Results: Despite the overall proteome similarities, there were significant differences in the expression of seven spots between the two groups (P <0.05). Three proteins, namely phosphatidylethanolaminebinding protein 1, proteasome activator complex subunit 1 and triosephosphate isomerase 1 were successfully identified by Mass technique and confirmed by western blot. All characterized proteins were over-expressed in CD4+ T cells from patients compared to CD4+ T cells from controls (P <0.05). Insilico analysis suggested that the over-expressed proteins interact with other proteins involved in cellular metabolism, especially glycolysis and ferroptosis pathway.

Conclusion: These findings suggest that metabolic adjustments in CD4+ T lymphocytes, which is in favor of increased glycolysis and Th2 differentiation are important in the context of PCOS.

Cite this article as:

Nasri Fatemeh , Zare Maryam, Doroudchi Mehrnoosh and Gharesi-Fard Behrouz *, Proteome Analysis of CD4⁺ T Cells Reveals Differentially Expressed Proteins in Infertile Polycystic Ovary Syndrome Patients, Endocrine, Metabolic & Immune Disorders - Drug Targets 2021; 21 (11) . https://dx.doi.org/10.2174/1871530320666201119152323