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当代阿耳茨海默病研究

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ISSN (Print): 1567-2050
ISSN (Online): 1875-5828

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Research Article

阿尔茨海默氏病患者外周血线粒体动态相关基因的变化

卷 17, 期 7, 2020

页: [616 - 625] 页: 10

弟呕挨: 10.2174/1567205017666201006162538

价格: $65

摘要

背景：线粒体功能障碍是一种病理特征，在阿尔茨海默氏病（AD）患者的大脑中较早出现。线粒体动力学的破坏导致线粒体的形态和功能受损。我们先前的研究表明，AD患者外周血中淀粉样β生成相关基因的表达发生了改变。 目的：本研究的目的是进一步研究与健康对照相比，AD患者外周血样品中涉及线粒体动力学（包括线粒体分裂和融合）和线粒体的线粒体基因的相对水平。 方法：通过实时聚合酶链反应分析mRNA水平。评价基因表达谱与认知能力的关系。 结果：观察到裂变相关基因的mRNA表达水平发生了显着变化。 AD受试者的Fission1（FIS1）水平明显高于健康对照者，而Dynamin相关蛋白1（DRP1）的表达则明显低于AD受试者。与健康的年轻对照组相比，AD受试者和老年对照组的线粒体相关基因，PTEN诱导的激酶1（PINK1）和微管相关蛋白1轻链3（LC3）的水平显着增加。在AD中，Parkin（PARK2）的mRNA水平显着降低。发现FIS1，DRP1和PARK2的表达水平与认知能力评分之间存在相关性。 结论：血液中线粒体动力学的改变可能反映了AD患者中枢和外周组织线粒体功能的损害。线粒体裂变以及线粒体基因图谱，可能是未来基于血液的AD生物标记物发展的潜在考虑因素。

关键词: 阿尔茨海默氏病，线粒体动力学，线粒体，FIS1，DRP1和衰老。

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