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Research Article

BMSCs Runx2的过表达改善膝盖软骨缺损的修复

卷 20, 期 5, 2020

页: [395 - 404] 页: 10

弟呕挨: 10.2174/1566523220666201005110339

价格: $65

摘要

背景：在关节软骨修复中，基因修饰的骨髓间充质干细胞（BMSC）被认为是单细胞注射的替代方法。 目的：本研究旨在探讨Runt相关转录因子2(Runx2)过表达骨髓间充质干细胞在体内的作用是否能提高兔模型膝关节软骨缺损修复组织的质量。 方法：将32只新西兰大白兔随机分为四组。空白组（Con）未注射任何东西，模型组（Mo）给予生理盐水，简单干细胞组（MSCs）接受MSCs注射，Runx2转染组（R-MSCs）接受Runx2过表达MSCs注射。适应环境一周后，在股内侧con的中央形成了直径为5 mm的圆柱形骨软骨缺损。在手术后的第一和第三周进行细胞和盐水注射。在第4和第8周通过肉眼和显微镜评估软骨修复。 结果：骨髓间充质干细胞组在第4周出现缺损，表面光滑。 显微镜下，R-MSCs组在8周的Masson三色染色中表现出与周围正常关节软骨组织相似的着色。 与R-MSCs相比，4周MSCs的COL-II、SOX9和AggrecanmRNA表达增强，8周表达降低，但仍高于Mo组水平(P<0.05)。 通过westernblot检查发现，4周时MSCs的COL-II和SOX9表达高于R-MSCs，8周时表达降低，但仍高于Mo水平(P<0.05)。 关节液中IL-1β含量也表明，R-MSCs修复软骨优于8周MSCs修复(P<0.05)。 结论：R-MSCs组表现出与周围正常关节软骨组织相似的细胞形态和排列，Runx2过表达的MSCs与MSCs相比，在8周时表现出整体上优越的软骨修复。

关键词: Runx2，膝关节软骨修复，骨髓间充质干细胞，软骨缺损，软骨缺损，动物模型。

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