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Anti-Infective Agents

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ISSN (Print): 2211-3525
ISSN (Online): 2211-3533

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Research Article

Antibiofilm Activity of ZnO/Zeolite Nanocomposite (ZnO/ZeoNC) Against Klebsiella pneumoniae and its Biocompatibility in an Animal Model

Author(s): Alireza Partoazar, Fatemeh Rahmani Bideskan, Nasrin Takzaree and Mohammad Mehdi Soltan Dallal*

Volume 19, Issue 2, 2021

Published on: 19 August, 2020

Page: [174 - 181] Pages: 8

DOI: 10.2174/2211352518999200819161229

Price: $65

Abstract

Background: Infectious diseases, whether intracellular or extracellular infections, biofilm- mediated, or medical device-associated, have always been a global public health problem, causing millions of deaths each year. The aim of this study was to evaluate the antibiofilm activity of ZnO/ZeoNC against K. pneumoniae along with the biocompatibility of the nanocomposite in vivo model.

Objective: The formation of biofilm by K. pneumoniae in the catheter-associated urinary tract causes a nosocomial infection. In this regard, antimicrobial nanomaterials have emerged as potent effective agents against biofilm formation. Nevertheless, nanoparticles have already been a challenge with possible side effects such as inflammation. The ZnO/ZeoNC may exhibit anti-biofilm property with minimal adverse effects.

Methods: The biofilm formation of K. pneumoniae strains was exposed to ZnO/ZeoNC and then SEM imaging was performed for morphological investigation of bacteria in biofilm state. The response to ZnO/ZeoNC embedded polyethylene tube of the tissue of mice was also analyzed during the 30-day experiment.

Results: The results of this study showed that ZnO/ZeoNC has significant antibiofilm activity against K. pneumoniae strains in its sublethal doses. The ZnO/ZeoNC also caused deformation in K. pneumoniae biofilm. In addition, ZnO/ZeoNC also reduced inflammatory response in cell tissue of rats subjected to polyethylene tube.

Conclusion: ZnO/ZeoNC can be used potentially against the infections caused by K. pneumonia biofilm without any irritability on the biotic surface such as the urinary tract.

Keywords: Anti-biofilm, nosocomial infection, nanoparticle, inflammation, urinary tract, biocompatibility.

Graphical Abstract

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