Construction and Evaluation of Peptide-Linked Lactobacillus brevis β-Galactosidase Heterodimers

Yuan-Yuan       Han; Hai-Yun       Yue; Xiao-Yang       Zhang; Yong-Mei       Lyu; Li       Liu; Josef       Voglmeir

doi:10.2174/0929866527666200813201242

Abstract

Background: β-galactosidases are enzymes that are utilized to hydrolyze lactose into galactose and glucose, and are is widely used in the food industry.

Objective: We describe the recombinant expression of an unstudied, heterodimeric β-galactosidase originating from Lactobacillus brevis ATCC 367 in Escherichia coli. Furthermore, six different constructs, in which the two protein subunits were fused with different peptide linkers, were also investigated.

Methods: The heterodimeric subunits of the β-galactosidase were cloned in expressed in various expression constructs, by using either two vectors for the independent expression of each subunit, or using a single Duet vector for the co-expression of the two subunits.

Results: The co-expression in two independent expression vectors only resulted in low β-galactosidase activities, whereas the co-expression in a single Duet vector of the independent and fused subunits increased the β-galactosidase activity significantly. The recombinant β-galactosidase showed comparable hydrolyzing properties towards lactose, N-acetyllactosamine, and pNP-β-D-galactoside.

Conclusion: The usability of the recombinant L. brevis β-galactosidase was further demonstrated by the hydrolysis of human, bovine, and goat milk samples. The herein presented fused β-galactosidase constructs may be of interest for analytical research as well as in food- and biotechnological applications.

Keywords: β-galactosidase, lactobacillus, fusion protein, peptide linkers, lactose hydrolysis, subunit co-expression.

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DOI https://dx.doi.org/10.2174/0929866527666200813201242	Print ISSN 0929-8665
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Protein & Peptide Letters

Construction and Evaluation of Peptide-Linked Lactobacillus brevis β-Galactosidase Heterodimers

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