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Current Pharmaceutical Biotechnology

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ISSN (Print): 1389-2010
ISSN (Online): 1873-4316

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Research Article

LC-MS/MS Determination of Apigenin in Rat Plasma and Application to Pharmacokinetic Study

Author(s): Shixing Zhu*, Jiayuan Zhang*, Zhihua Lv and Mingming Yu

Volume 22, Issue 2, 2021

Published on: 07 August, 2020

Page: [274 - 280] Pages: 7

DOI: 10.2174/1389201021666200807113144

Price: $65

Abstract

Background: Apigenin, a natural plant flavone, has been shown to possess a variety of biological properties.

Objective: In this report, a highly selective and sensitive LC-MS/MS method was developed and validated for the determination of apigenin in rat plasma.

Methods: Analysts were separated on the HSS T3 column (1.8 μm 2.1×100 mm) using acetonitrile and 0.1% formic acid in 2mM ammonium acetate buffer at a supply rate of 0.200 mL/min as eluent in gradient model.

Results: Plasma samples were treated by protein precipitation using acetonitrile for the recovery ranging from 86.5% to 90.1% for apigenin. The calibration curves followed linearity in the concentration range of 0.50-500ng/mL. The inter-day and intra-day precisions at different QC levels within 13.1% and the accuracies ranged from -10.6% to 8.6%.

Conclusion: The assay has been successfully applied to the pharmacokinetic study of apigenin in rats.

Keywords: Apigenin, LC-MS/MS, pharmacokinetic study, rats, validation, plant flavone.

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[1]
Shukla, S.; Gupta, S. Apigenin: A promising molecule for cancer prevention. Pharm. Res., 2010, 27(6), 962-978.
[http://dx.doi.org/10.1007/s11095-010-0089-7] [PMID: 20306120]
[2]
Ali, F.; Naz, F.; Jyoti, S.; Siddique, Y.H.; Ali, F.; Naz, F. Health functionality of apigenin: A review. Int. J. Food Prop., 2017, 20, 1197-1238.
[http://dx.doi.org/10.1080/10942912.2016.1207188]
[3]
Yan, X.; Qi, M.; Li, P.; Zhan, Y.; Shao, H. Apigenin in cancer therapy: Anti-cancer effects and mechanisms of action. Cell Biosci., 2017, 7, 50.
[http://dx.doi.org/10.1186/s13578-017-0179-x] [PMID: 29034071]
[4]
Patel, D.; Shukla, S.; Gupta, S. Apigenin and cancer chemoprevention: Progress, potential and promise (review) Int. J. Oncol., 2007, 30(1), 233-245.
[http://dx.doi.org/10.3892/ijo.30.1.233] [PMID: 17143534]
[5]
Madunić, J.; Madunić, I.V.; Gajski, G.; Popić, J.; Garaj-Vrhovac, V. Apigenin: A dietary flavonoid with diverse anticancer properties. Cancer Lett., 2018, 413, 11-22.
[http://dx.doi.org/10.1016/j.canlet.2017.10.041] [PMID: 29097249]
[6]
Wang, J.; Liu, Y.T.; Xiao, L.; Zhu, L.; Wang, Q.; Yan, T. Anti-inflammatory effects of apigenin in lipopolysaccharide-induced inflammatory in acute lung injury by suppressing COX-2 and NF-kB pathway. Inflammation, 2014, 37(6), 2085-2090.
[http://dx.doi.org/10.1007/s10753-014-9942-x] [PMID: 24958013]
[7]
An, F.; Wang, S.; Yuan, D.; Gong, Y.; Wang, S. Attenuation of oxidative stress of erythrocytes by plant-derived flavonoids, orientin and luteolin. Evid. Based Complement. Alternat. Med., 2016, 2016, 3401269.
[http://dx.doi.org/10.1155/2016/3401269] [PMID: 26966458]
[8]
Weng, L.; Guo, X.; Li, Y.; Yang, X.; Han, Y. Apigenin reverses depression-like behavior induced by chronic corticosterone treatment in mice. Eur. J. Pharmacol., 2016, 774, 50-54.
[http://dx.doi.org/10.1016/j.ejphar.2016.01.015] [PMID: 26826594]
[9]
Lim, H.; Park, H.; Kim, H.P. Effects of flavonoids on senescence-associated secretory phenotype formation from bleomycin-induced senescence in BJ fibroblasts. Biochem. Pharmacol., 2015, 96(4), 337-348.
[http://dx.doi.org/10.1016/j.bcp.2015.06.013] [PMID: 26093063]
[10]
Elzayat, E.M.; Shakeel, F.; Alshehri, S.; Ibrahim, M.A.; Altamimi, M.A.; Kazi, M.; Alanazi, F.K.; Haq, N. UHPLC assisted simultaneous separation of apigenin and prednisolone and its application in the pharmacokinetics of apigenin. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2019, 1117, 58-65.
[http://dx.doi.org/10.1016/j.jchromb.2019.04.006] [PMID: 30999274]
[11]
Dai, B.; Hu, Z.; Li, H.; Yan, C.; Zhang, L. Simultaneous determination of six flavonoids from Paulownia tomentosa flower extract in rat plasma by LC-MS/MS and its application to a pharmacokinetic study. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2015, 978-979, 54-61.
[http://dx.doi.org/10.1016/j.jchromb.2014.11.021] [PMID: 25531870]
[12]
Sun, D.; Dong, L.; Guo, P.; Yan, W.; Wang, C.; Zhang, Z. Simultaneous determination of four flavonoids and one phenolic acid in rat plasma by LC-MS/MS and its application to a pharmacokinetic study after oral administration of the Herba Desmodii styracifolii extract. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2013, 932, 66-73.
[http://dx.doi.org/10.1016/j.jchromb.2013.06.001] [PMID: 23831698]
[13]
Tao, Y.; Su, D.; Li, W.; Cai, B. Pharmacokinetic comparisons of six components from raw and vinegar-processed Daphne genkwa aqueous extracts following oral administration in rats by employing UHPLC-MS/MS approaches. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2018, 1079, 34-40.
[http://dx.doi.org/10.1016/j.jchromb.2018.02.005] [PMID: 29428673]
[14]
Wan, L.; Guo, C.; Yu, Q.; Li, Y.; Wang, X.; Wang, X.; Chen, C. Quantitative determination of apigenin and its metabolism in rat plasma after intravenous bolus administration by HPLC coupled with tandem mass spectrometry. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2007, 855(2), 286-289.
[http://dx.doi.org/10.1016/j.jchromb.2007.05.007] [PMID: 17561454]
[15]
Duan, K.; Yuan, Z.; Guo, W.; Meng, Y.; Cui, Y.; Kong, D.; Zhang, L.; Wang, N. LC-MS/MS determination and pharmacokinetic study of five flavone components after solvent extraction/acid hydrolysis in rat plasma after oral administration of Verbena officinalis L. extract. J. Ethnopharmacol., 2011, 135(2), 201-208.
[http://dx.doi.org/10.1016/j.jep.2011.01.002] [PMID: 21220003]
[16]
Wang, T.; Xiao, J.; Hou, H.; Li, P.; Yuan, Z.; Xu, H.; Liu, R.; Li, Q.; Bi, K. Development of an ultra-fast liquid chromatography-tandem mass spectrometry method for simultaneous determination of seven flavonoids in rat plasma: Application to a comparative pharmacokinetic investigation of Ginkgo biloba extract and single pure ginkgo flavonoids after oral administration. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2017, 1060, 173-181.
[http://dx.doi.org/10.1016/j.jchromb.2017.05.021] [PMID: 28622621]
[17]
Cheruvu, H.S.; Yadav, N.K.; Valicherla, G.R.; Arya, R.K.; Hussain, Z.; Sharma, C.; Arya, K.R.; Singh, R.K.; Datta, D.; Gayen, J.R. LC-MS/MS method for the simultaneous quantification of luteolin, wedelolactone and apigenin in mice plasma using hansen solubility parameters for liquid-liquid extraction: Application to pharmacokinetics of Eclipta alba chloroform fraction. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2018, 1081-1082, 76-86.
[http://dx.doi.org/10.1016/j.jchromb.2018.01.035] [PMID: 29518720]
[18]
Li, L.P.; Jiang, H.D. Determination and assay validation of luteolin and apigenin in human urine after oral administration of tablet of Chrysanthemum morifolium extract by HPLC. J. Pharm. Biomed. Anal., 2006, 41(1), 261-265.
[http://dx.doi.org/10.1016/j.jpba.2005.10.019] [PMID: 16318906]
[19]
Li, L.; Jiang, H.; Wu, H.; Zeng, S. Simultaneous determination of luteolin and apigenin in dog plasma by RP-HPLC. J. Pharm. Biomed. Anal., 2005, 37(3), 615-620.
[http://dx.doi.org/10.1016/j.jpba.2004.11.012] [PMID: 15740925]
[20]
Yu, M.; Abdallah, I.A.; Shin, S.H.; Hammell, D.C.; Stinchcomb, A.L.; Hassan, H.E. LC-MS determination of fentanyl in human serum and application to a fentanyl transdermal delivery pharmacokinetic study. Bioanalysis, 2017, 9(20), 1551-1560.
[http://dx.doi.org/10.4155/bio-2017-0174] [PMID: 28914084]
[21]
FDA. Bioanalytical Method Validation Guidance., 2018.
[22]
Tang, D.; Chen, K.; Huang, L.; Li, J. Pharmacokinetic properties and drug interactions of apigenin, a natural flavone. Expert Opin. Drug Metab. Toxicol., 2017, 13(3), 323-330.
[http://dx.doi.org/10.1080/17425255.2017.1251903] [PMID: 27766890]

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