Login Register Cart 0
Generic placeholder image

Current Biotechnology

Editor-in-Chief

ISSN (Print): 2211-5501
ISSN (Online): 2211-551X

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Isolation and Characterization of Halophilic and Halotolerant Bacteria from Urmia Lake after the Recent Drought Disaster in 2015

Author(s): Elham Kazemi, Vahideh Tarhriz, Mohammad S. Hejazi* and Mohammad A. Amoozegar*

Volume 9, Issue 2, 2020

Page: [111 - 119] Pages: 9

DOI: 10.2174/2211550109999200802153647

Price: $65

Abstract

Background: Due to the last two decades of drought disaster, which resulted in the loss of the main part of Urmia Lake water and changed the natural conditions of an environment, especially ionic strength.

Objective: We aimed to isolate and characterize halophilic and halotolerant bacteria in Urmia Lake, Iran, 2015. Urmia Lake is a permanent and salty inland lake located in the Azerbaijan region in northwestern Iran.

Methods: Sampling was carried out in multiple water-filled locations of the lake. Liquid basal media for the enrichment of bacteria was successively applied and colonies were isolated by the plating method. Isolates were then distinguished based on differences in colony, Gram staining, microscopic shape, and biochemical properties.

Results: One chemolithotrophic isolate belonging to Thiobacillus thioparus and 41 heterotrophic isolates were obtained. The 16S rRNA gene sequences showed that all 42 isolates belong to the genera Kocuria (21.42%), Marinobacter (11.90%), Micrococcus (11.90%), Thalassobacillus (11.90%), Bacillus (11.90%), Halomonas (7.14%) and Thiobacillus (2.38%).

Conclusion: Based on 16S rRNA similarity, 5 of 41 isolates showed the potential to be introduced as new species. The dominant genera with abounded frequency were found to be Kocuria, Bacillus and Thalassobacillus genera.

Keywords: Aquatic bacteria, chemolithotrophic, Kocuria, Thalassobacillus, halotolerant bacteria, halophilic bacteria.

« Previous Next »
Graphical Abstract

[1]
Rampelotto PH. Resistance of microorganisms to extreme environmental conditions and its contribution to astrobiology. Sustainability 2010; 2(6): 1602-23.
[http://dx.doi.org/10.3390/su2061602]
[2]
Hamidi-Razi H, Mazaheri M, Carvajalino-Fernández M, Vali-Samani J. Investigating the restoration of Lake Urmia using a numerical modelling approach. J Great Lakes Res 2019; 45(1): 87-97.
[http://dx.doi.org/10.1016/j.jglr.2018.10.002]
[3]
Passey BH, Ji H. Triple oxygen isotope signatures of evaporation in lake waters and carbonates: A case study from the western United States. Earth Planet Sci Lett 2019; 518: 1-12.
[http://dx.doi.org/10.1016/j.epsl.2019.04.026]
[4]
Caton TM, Witte LR, Ngyuen HD, Buchheim JA, Buchheim MA, Schneegurt MA. Halotolerant aerobic heterotrophic bacteria from the Great Salt Plains of Oklahoma. Microb Ecol 2004; 48(4): 449-62.
[http://dx.doi.org/10.1007/s00248-004-0211-7] [PMID: 15696379]
[5]
Eimanifar A, Mohebbi F. Urmia Lake (Northwest Iran): A brief review. Saline Syst 2007; 3(1): 5.
[http://dx.doi.org/10.1186/1746-1448-3-5] [PMID: 17506897]
[6]
Safarpour A, Amoozegar MA, Ventosa A. Hypersaline environments of Iran: Prokaryotic biodiversity and their potentials in microbial biotechnology. In: Egamberdieva D, Birkeland N-K, Panosyan H, Li W-J Extremophiles in eurasian ecosystems: Ecology, diversity, and applications. Springer 2018; pp. 265-98.
[http://dx.doi.org/10.1007/978-981-13-0329-6]
[7]
Anand K, Karuppanapandian T, Sinha PB. Isolation, identification and characterization of fungi from Vinoba Bhave University Campus, Hazaribag District innovation in materials science and engineering. Springer 2019; pp. 115-29.
[8]
Bonnaud EM, Troupin C, Dacheux L, et al. Comparison of intra- and inter-host genetic diversity in rabies virus during experimental cross-species transmission. PLoS Pathog 2019; 15(6)e1007799
[http://dx.doi.org/10.1371/journal.ppat.1007799] [PMID: 31220188]
[9]
Davies ZG, Dallimer M, Fisher JC, Fuller RA. Biodiversity and health: Implications for conservation biodiversity and health in the face of climate change. Springer 2019; pp. 283-94.
[http://dx.doi.org/10.1007/978-3-030-02318-8_12]
[10]
Tarhriz V, Eyvazi S, Shakeri E, Hejazi MS, Dilmaghani A. Antibacterial and antifungal activity of novel freshwater bacterium “tabrizicola aquatica “as a prominent natural antibiotic available in grügol lake. Pharma Sci 2019; 25(3)
[11]
Tarhriz V, Akbari Z, Dilmaghani A, Hamidi A, Hejazi MA, Hejazi MS. Bioreduction of iron and biosorption of heavy metals (Ni2+, Co2+, Pb2+) by a novel environmental bacterium, Tabrizicola aquatica rcri19 t. Asian J Water Environ Pollut 2019; 16(3): 73-81.
[http://dx.doi.org/10.3233/AJW190035]
[12]
Singh P, Jain K, Desai C, Tiwari O, Madamwar D. Microbial community dynamics of extremophiles/extreme environment microbial diversity in the genomic era. Elsevier 2019; pp. 323-32.
[http://dx.doi.org/10.1016/B978-0-12-814849-5.00018-6]
[13]
Shadi M, Heydari H, Elyasifar B, Dilmaghani A. Isolation and identification of hydrolytic enzymes generated by halophilic bacteria in center of Iran 2019.
[14]
Antranikian G, Vorgias CE, Bertoldo C. Extreme environments as a resource for microorganisms and novel biocatalysts Marine biotechnology I. Springer 2005; pp. 219-62.
[15]
Elyasifar B, Jafari S, Hallaj-Nezhadi S, Chapeland-leclerc F, Ruprich-Robert G, Dilmaghani A. Isolation and identification of antibiotic-producing halophilic bacteria from dagh biarjmand and haj aligholi salt deserts, Iran. Pharm Sci 2019; 25(1): 70-7.
[http://dx.doi.org/10.15171/PS.2019.11]
[16]
Roberts MF. Organic compatible solutes of halotolerant and halophilic microorganisms. Saline Syst 2005; 1(1): 5.
[http://dx.doi.org/10.1186/1746-1448-1-5] [PMID: 16176595]
[17]
Rahman SS, Siddique R, Tabassum N. Isolation and identification of halotolerant soil bacteria from coastal Patenga area. BMC Res Notes 2017; 10(1): 531.
[http://dx.doi.org/10.1186/s13104-017-2855-7] [PMID: 29084602]
[18]
Zahran H. Diversity, adaptation and activity of the bacterial flora in saline environments. Biol Fertil Soils 1997; 25(3): 211-23.
[http://dx.doi.org/10.1007/s003740050306]
[19]
Kaur A, Chaudhary A, Kaur A, Choudhary R, Kaushik R. Phospholipid fatty acid–a bioindicator of environment monitoring and assessment in soil ecosystem. Curr Sci 2005; 89(7): 1103-12.
[20]
Barin M, Aliasgharzad N, Olsson PA, Rasouli-Sadaghiani M. Salinity-induced differences in soil microbial communities around the hypersaline Lake Urmia. Soil Res 2015; 53(5): 494-504.
[http://dx.doi.org/10.1071/SR14090]
[21]
Ahmady-Birgani H, Ravan P, Schlosser JS, Cuevas-Robles A. AzadiAghdam M, Sorooshian A. On the chemical nature of wet deposition over a major desiccated lake: Case study for Lake Urmia basin. Atmos Res 2020.234104762
[http://dx.doi.org/10.1016/j.atmosres.2019.104762]
[22]
Vahed SZ, Forouhandeh H, Hassanzadeh S, Klenk H-P, Hejazi MA, Hejazi MS. Isolation and characterization of halophilic bacteria from Urmia Lake in Iran. Mikrobiologiia 2011; 80(6): 826-33.
[PMID: 22393767]
[23]
Vahed SZ, Forouhandeh H, Tarhriz V, et al. Halomonas tabrizica sp. nov., a novel moderately halophilic bacterium isolated from Urmia Lake in Iran. Antonie van Leeuwenhoek 2018; 111(7): 1139-48.
[http://dx.doi.org/10.1007/s10482-018-1018-8] [PMID: 29376208]
[24]
Khan SA, Zununi Vahed S, Forouhandeh H, et al. Halomonas urmiana sp. nov., a moderately halophilic bacterium isolated from Urmia Lake in Iran. Int J Syst Evol Microbiol 2020; 70(4): 2254-60.
[http://dx.doi.org/10.1099/ijsem.0.004005] [PMID: 32039745]
[25]
Anderson R, Berges R, Harrison P, Watanabe M. Enriched natural seawater media. In: Appendix A—Recipes for freshwater and seawater media. Burlington, USA: Algal Culturing Techniques. Elsevier Academic Press 2005; p. 507.
[26]
Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961; 3(2): 208-IN1.
[http://dx.doi.org/10.1016/S0022-2836(61)80047-8]
[27]
Wu X-Y, Shi K-L, Xu X-W, Wu M, Oren A, Zhu X-F. Alkaliphilus halophilus sp. nov., a strictly anaerobic and halophilic bacterium isolated from a saline lake, and emended description of the genus Alkaliphilus. Int J Syst Evol Microbiol 2010; 60(Pt 12): 2898-902.
[http://dx.doi.org/10.1099/ijs.0.014084-0] [PMID: 20097801]
[28]
Tarhriz V, Mohammadzadeh F, Hejazi MS, Nematzadeh G, Rahimi E. Isolation and characterization of some aquatic bacteria from Qurugol Lake in Azerbaijan under aerobic conditions. Adv Environ Biol 2011; 5(10): 3173-9.
[29]
Larkin MA, Blackshields G, Brown N, et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007; 23(21): 2947-8.
[30]
Saitou N, Nei M. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4(4): 406-25.
[PMID: 3447015]
[31]
Felsenstein J. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 1985; 39(4): 783-91.
[http://dx.doi.org/10.1111/j.1558-5646.1985.tb00420.x PMID: 28561359]
[32]
Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA 2004; 101(30): 11030-5.
[http://dx.doi.org/10.1073/pnas.0404206101] [PMID: 15258291]
[33]
Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35(6): 1547-9.
[http://dx.doi.org/10.1093/molbev/msy096] [PMID: 29722887]
[34]
Murray R. Determinative and cytological light microscopy. In: Methods for general and molecular bacteriology. 1994; pp. 7-20.
[35]
MacFaddin JF. Individual biochemical tests.Biochemical tests for identification of medical bacteria. 3rd ed. Philadelphia: Lippincott Williams & Wilkins 2000; pp. 3-453.
[36]
Alain K, Querellou J. Cultivating the uncultured: Limits, advances and future challenges. Extremophiles 2009; 13(4): 583-94.
[http://dx.doi.org/10.1007/s00792-009-0261-3] [PMID: 19548063]
[37]
Hughes JB, Hellmann JJ, Ricketts TH, Bohannan BJ. Counting the uncountable: Statistical approaches to estimating microbial diversity. Appl Environ Microbiol 2001; 67(10): 4399-406.
[http://dx.doi.org/10.1128/AEM.67.10.4399-4406.2001 PMID: 11571135]
[38]
Mirchi A, Madani K, Aghakouchak A. Lake Urmia: How Iran's most famous lake is disappearing The Guardian 2015.
[39]
Kashi FJ, Owlia P, Amoozegar MA, Yakhchali B, Kazemi B. Diversity of cultivable microorganisms in the eastern part of Urmia salt lake, Iran. J Microbiol Biotechnol Food Sci 2014; 4(1): 36.
[http://dx.doi.org/10.15414/jmbfs.2014.4.1.36-43]
[40]
Kellermann C, Griebler C. Thiobacillus thiophilus sp. nov., a chemolithoautotrophic, thiosulfate-oxidizing bacterium isolated from contaminated aquifer sediments. Int J Syst Evol Microbiol 2009; 59(Pt 3): 583-8.
[http://dx.doi.org/10.1099/ijs.0.002808-0] [PMID: 19244446]
[41]
Makhdoumi-Kakhki A, Amoozegar MA, Kazemi B, Pašić L, Ventosa A. Prokaryotic diversity in Aran-Bidgol salt lake, the largest hypersaline playa in Iran. Microbes Environ 2012; 27(1): 87-93.
[http://dx.doi.org/10.1264/jsme2.me11267] [PMID: 22185719]
[42]
Steven B, Briggs G, McKay CP, Pollard WH, Greer CW, Whyte LG. Characterization of the microbial diversity in a permafrost sample from the Canadian high Arctic using culture-dependent and culture-independent methods. FEMS Microbiol Ecol 2007; 59(2): 513-23.
[http://dx.doi.org/10.1111/j.1574-6941.2006.00247.x PMID: 17313587]
[43]
Selvarajan R, Sibanda T, Tekere M, Nyoni H, Meddows-Taylor S. Diversity analysis and bioresource characterization of halophilic bacteria isolated from a South African saltpan. Molecules 2017; 22(4): 657.
[http://dx.doi.org/10.3390/molecules22040657] [PMID: 28425950]
[44]
Vitorino LC, Bessa LA. Technological microbiology: Development and applications. Front Microbiol 2017; 8: 827.
[http://dx.doi.org/10.3389/fmicb.2017.00827] [PMID: 28539920]

Rights & Permissions Print Cite
Article Metrics
4
© 2024 Bentham Science Publishers | Privacy Policy