摘要
背景:严重烧伤的休克阶段是预后的关键决定因素,是引发全身炎症反应综合征和多器官衰竭的关键。已知含有丰富 miRNA 的细胞外囊泡 (EV) 参与各种生物过程。由于缺乏对严重烧伤中 miRNA 变化的研究,我们的研究分析了休克阶段严重烧伤中 EV 的 miRNA 谱。 方法:从重度烧伤大鼠(体表面积的30%,III°)血清中提取EVs,采用二代测序法检测血清EVs中miRNAs的表达。使用GO Ontology(GO)和Kyoto Encyclopedia of Genes and Genomes(KEGG)对显着差异表达(DE)的miRNA的靶基因进行功能分析。 结果:在严重烧伤休克早期鉴定出 34 个 DE miRNA,在严重烧伤休克晚期鉴定出 63 个。此外,miRNA-339-5p、miRNA-1、miRNA-382-5p、miRNA134-5p、miRNA-133a-5p 和 miRNA-365a-5p 在整个休克阶段都是 DE,基于 P < 0.01 和 | log2 (foldchange)| ≥ 1 个标准。 GO和KEGG分析显示DE miRNA的靶基因主要富集代谢过程、免疫系统过程和信号通路。 结论:据我们所知,这是第一项证明从休克阶段严重烧伤血清中分离出的 EV 的 miRNA 表达谱的研究。下调和上调之间存在显着差异。因此,miRNAs 有可能成为严重烧伤并发症的新型生物标志物。
关键词: 细胞外囊泡、miRNA、严重烧伤、休克阶段、功能富集分析、KEGG。
Current Molecular Medicine
Title:Altered Expression of microRNAs in Serum Extracellular Vesicles in Rats with Severe Burns during Shock Stage
Volume: 21 Issue: 8
关键词: 细胞外囊泡、miRNA、严重烧伤、休克阶段、功能富集分析、KEGG。
摘要:
Background: The shock stage of severe burns is a critical determinant of prognosis and the induction of systemic inflammatory response syndrome and multiple organ failure. Extracellular vesicles (EVs) containing abundant miRNAs are known to participate in various biological processes. Due to the lack of research on alternations of miRNAs in severe burns, our study analyzed the miRNA profiles of EVs in severe burns during the shock stage.
Methods: EVs were extracted from the serum of rats with severe burns (30% of total body surface area, III°), and the expression of miRNAs in serum EVs was determined by next-generation sequencing. Functional analysis of target genes of miRNAs that were significantly differentially expressed (DE) was performed using GO Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG).
Results: Thirty-four DE miRNAs were identified at the early stage of severe burn shock and 63 at the late stage of severe burn shock. In addition, miRNA-339-5p, miRNA-1, miRNA-382-5p, miRNA134-5p, miRNA-133a-5p, and miRNA-365a-5p were DE throughout the entire shock stage, based on P < 0.01 and |log2 (foldchange)| ≥ 1 criteria. GO and KEGG analysis revealed that the target genes of DE miRNAs mainly enriched the metabolic process, immune system processes, and signal pathways.
Conclusion: To our best knowledge, this is the first study demonstrating the miRNA expression profiles of EVs isolated from serum with severe burns during the shock stage. There are significant differences between downregulation and upregulation. Thus, miRNAs have the potential for novel biomarkers for the complication of severe burns.
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Cite this article as:
Altered Expression of microRNAs in Serum Extracellular Vesicles in Rats with Severe Burns during Shock Stage, Current Molecular Medicine 2021; 21 (8) . https://dx.doi.org/10.2174/1566524020666200712181644
DOI https://dx.doi.org/10.2174/1566524020666200712181644 |
Print ISSN 1566-5240 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5666 |
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