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Current Topics in Medicinal Chemistry

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ISSN (Print): 1568-0266
ISSN (Online): 1873-4294

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Research Article

Identification and Characterization of Lipopeptide Biosurfactant Producing Microbacterium sp Isolated from Brackish River Water

Author(s): Anuraj Nayarisseri*, Ravina Khandelwal and Sanjeev Kumar Singh*

Volume 20, Issue 24, 2020

Page: [2221 - 2234] Pages: 14

DOI: 10.2174/1568026620666200628144716

Price: $65

Abstract

Background: Bioremediation has taken its call for removing pollutants for years. The oilcontaminated surroundings are majorly hazardous for sustaining life, but a great contribution to nature in the form of microorganisms. The complex carbon-hydrogen chain has served as classic raw material to chemical industries, which has perked up the hydrocarbon waste. Microbial remediation has been thus, focused to deal with the lacuna, where the new addition to this category is Microbacterium species.

Objectives: The identification and characterization of lipopeptide biosurfactant producing Microbacterium spp. isolated from brackish river water.

Methods: The strain was isolated from an oil-contaminated lake. The strain was tested with all the other isolated species for oil degradation using screening protocols such as haemolysis, oil spread assay, BATH, E24, etc. The produced biosurfactant was extracted by acid precipitation, followed by solvent recovery. The strain with maximum potential was sequenced and was subjected to phylogeny assessment using in silico tools.

Results: Novel Microbacterium species produce the extracellular biosurfactant. The surface tension of Microbacterium was found to be 32mN/m, indicates its powerful surface tension-reducing property. The strain was optimized for the production of biosurfactant and the best results were obtained with sucrose (2%) and yeast extract (3%) medium at 7 pH and 40°C temperature.

Conclusion: The isolate was confirmed to be a novel Microbacterium species that could produce 0.461 gm biosurfactant in 100 ml of the medium throughout a life cycle and novel strain of isolate was deposited to NCBI as Microbacterium spp. ANSKSLAB01 using an accession number: KU179507.

Keywords: Biosurfactant producing bacteria, Microbacterium, 16S rRNA sequencing, Phylogenetic analysis, Lipopeptide biosurfactant, Hydrocarbon.

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[1]
Sarkar, A. A critical evaluation of MEOR processes. In Situ, 1989, 13(4), 207-238.
[2]
Cooper, D.; Zajic, J. Surface-active compounds from microorganisms. Adv. Appl. Microbiol., 1980, 26(229), 53.
[3]
Cooper, D.G. Biosurfactants. Microbiol. Sci., 1986, 3(5), 145-149.
[PMID: 3153155]
[4]
Cooper, D.G.; Paddock, D.A. Torulopsis petrophilum and surface activity. Appl. Environ. Microbiol., 1983, 46(6), 1426-1429.
[http://dx.doi.org/10.1128/AEM.46.6.1426-1429.1983 ] [PMID: 16346449]
[5]
Cooper, D.; Zajic, J.; Denis, C. Surface active properties of a biosurfactant fromCorynebacterium lepus. J. Am. Oil Chem. Soc., 1981, 58(1), 77-80.
[http://dx.doi.org/10.1007/BF02666059]
[6]
Anvari, S. Physiochemical and thermodynamic characterization of lipopeptide biosurfactant secreted by Bacillus tequilensis HK01. RSC Advances, 2015, 5(111), 91836-91845.
[http://dx.doi.org/10.1039/C5RA17275F]
[7]
Cortés-Camargo, S. Production of biosurfactants from vine-trimming shoots using the halotolerant strain Bacillus tequilensis ZSB10. Ind. Crops Prod., 2016, 79, 258-266.
[http://dx.doi.org/10.1016/j.indcrop.2015.11.003]
[8]
Shoeb, E. Screening and characterization of biosurfactant-producing bacteria isolated from the Arabian Sea coast of Karachi. Turk. J. Biol., 2015, 39(2), 210-216.
[http://dx.doi.org/10.3906/biy-1405-63]
[9]
Abouseoud, M. Evaluation of different carbon and nitrogen sources in production of biosurfactant by Pseudomonas fluorescens. Desalination, 2008, 223(1), 143-151.
[http://dx.doi.org/10.1016/j.desal.2007.01.198]
[10]
Hazra, C.; Kundu, D.; Chaudhari, A. Lipopeptide biosurfactant from Bacillus clausii BS02 using sunflower oil soapstock: evaluation of high throughput screening methods, production, purification, characterization and its insecticidal activity. RSC Advances, 2015, 5(4), 2974-2982.
[http://dx.doi.org/10.1039/C4RA13261K]
[11]
Padmavathi, A.R.; Pandian, S.K. Antibiofilm activity of biosurfactant producing coral associated bacteria isolated from gulf of mannar. Indian J. Microbiol., 2014, 54(4), 376-382.
[http://dx.doi.org/10.1007/s12088-014-0474-8 ] [PMID: 25320434]
[12]
Funke, G.; Falsen, E.; Barreau, C. Primary identification of Microbacterium spp. encountered in clinical specimens as CDC coryneform group A-4 and A-5 bacteria. J. Clin. Microbiol., 1995, 33(1), 188-192.
[http://dx.doi.org/10.1128/JCM.33.1.188-192.1995 ] [PMID: 7699039]
[13]
Collins, M.D.; Jones, D.; Kroppenstedt, R.M. Reclassification of Brevibacterium imperiale (Steinhaus) and “Corynebacterium laevaniformans” (Dias and Bhat) in a Redefined Genus Microbacterium (Orla-Jensen), as Microbacterium imperiale comb. nov. and Microbacterium laevaniformans nom. rev.; comb. nov. Syst. Appl. Microbiol., 1983, 4(1), 65-78.
[http://dx.doi.org/10.1016/S0723-2020(83)80034-4 ] [PMID: 23196300]
[14]
Nayarisseri, A.; Singh, P.; Singh, S.K. Screening, isolation and characterization of biosurfactant producing Bacillus subtilis strain ANSKLAB03. Bioinformation, 2018, 14(6), 304-314.
[http://dx.doi.org/10.6026/97320630014304 ] [PMID: 30237676]
[15]
Liang, T-W.; Wu, C.C.; Cheng, W.T.; Chen, Y.C.; Wang, C.L.; Wang, I.L.; Wang, S.L. Exopolysaccharides and antimicrobial biosurfactants produced by Paenibacillus macerans TKU029. Appl. Biochem. Biotechnol., 2014, 172(2), 933-950.
[http://dx.doi.org/10.1007/s12010-013-0568-5 ] [PMID: 24122708]
[16]
Karanth, N.; Deo, P.; Veenanadig, N. Microbial production of biosurfactants and their importance. Curr. Sci., 1999, 77(1), 116-126.
[17]
Rahman, P.K.; Gakpe, E. Production, characterisation and applications of biosurfactants-Review. Biotechnology (Faisalabad), 2008, 7(2), 360-370.
[http://dx.doi.org/10.3923/biotech.2008.360.370]
[18]
Muthusamy, K. Biosurfactants: Properties, commercial production and application. Current Science (00113891), 2008, 94(6), 736-747.
[19]
Menezes Bento, F.; de Oliveira Camargo, F.A.; Okeke, B.C.; Frankenberger, W.T., Jr Diversity of biosurfactant producing microorganisms isolated from soils contaminated with diesel oil. Microbiol. Res., 2005, 160(3), 249-255.
[http://dx.doi.org/10.1016/j.micres.2004.08.005 ] [PMID: 16035236]
[20]
Mercade, M. Screening and selection of surfactant‐producing bacteria from waste lubricating oil. J. Appl. Bacteriol., 1996, 81(2), 161-166.
[http://dx.doi.org/10.1111/j.1365-2672.1996.tb04494.x]
[21]
Yan, S. Characterization of oil-degrading bacteria from oil-contaminated soil and activity of their enzymes. Biotechnol. Biotechnol. Equip., 2013, 27(4), 3932-3938.
[http://dx.doi.org/10.5504/BBEQ.2013.0050]
[22]
Saravanan, V.; Vijayakumar, S. Isolation and screening of biosurfactant producing microorganisms from oil contaminated soil. J. Acad. Indus. Res, 2012, 1(5), 264-268.
[23]
Kavuthodi, B.; Thomas, S.K.; Sebastian, D. Co-production of Pectinase and Biosurfactant by the Newly Isolated Strain Bacillus subtilis BKDS1. Br. Microbiol. Res. J., 2015, 10(2), 1-12.
[http://dx.doi.org/10.9734/BMRJ/2015/19627]
[24]
Van der Vegt, W. Assessment of bacterial biosurfactant production through axisymmetric drop shape analysis by profile. Appl. Microbiol. Biotechnol., 1991, 35(6), 766-770.
[http://dx.doi.org/10.1007/BF00169892]
[25]
Thavasi, R.; Sharma, S.; Jayalakshmi, S. Evaluation of screening methods for the isolation of biosurfactant producing marine bacteria. J. Pet. Environ. Biotechnol., 2011.
[26]
Walter, V.; Syldatk, C.; Hausmann, R. Screening concepts for the isolation of biosurfactant producing microorganisms. In: Biosurfactants; Springer: Berlin, 2010; pp. 1-13.
[http://dx.doi.org/10.1007/978-1-4419-5979-9_1]
[27]
Batista, S.B.; Mounteer, A.H.; Amorim, F.R.; Tótola, M.R. Isolation and characterization of biosurfactant/bioemulsifier-producing bacteria from petroleum contaminated sites. Bioresour. Technol., 2006, 97(6), 868-875.
[http://dx.doi.org/10.1016/j.biortech.2005.04.020 ] [PMID: 15951168]
[28]
Carrillo, P.G.; Mardaraz, C.; Pitta-Alvarez, S.I.; Giulietti, A.M. Isolation and selection of biosurfactant-producing bacteria. World J. Microbiol. Biotechnol., 1996, 12(1), 82-84.
[http://dx.doi.org/10.1007/BF00327807 ] [PMID: 24415095]
[29]
Morikawa, M.; Hirata, Y.; Imanaka, T. A study on the structure-function relationship of lipopeptide biosurfactants. Biochim. Biophys. Acta, 2000, 1488(3), 211-218.
[http://dx.doi.org/10.1016/S1388-1981(00)00124-4 ] [PMID: 11082531]
[30]
Bodour, A.A.; Miller-Maier, R.M. Application of a modified drop-collapse technique for surfactant quantitation and screening of biosurfactant-producing microorganisms. J. Microbiol. Methods, 1998, 32(3), 273-280.
[http://dx.doi.org/10.1016/S0167-7012(98)00031-1]
[31]
Youssef, N.H.; Duncan, K.E.; Nagle, D.P.; Savage, K.N.; Knapp, R.M.; McInerney, M.J. Comparison of methods to detect biosurfactant production by diverse microorganisms. J. Microbiol. Methods, 2004, 56(3), 339-347.
[http://dx.doi.org/10.1016/j.mimet.2003.11.001 ] [PMID: 14967225]
[32]
Rosenberg, M.; Gutnick, D.; Rosenberg, E. Adherence of bacteria to hydrocarbons: a simple method for measuring cell‐surface hydrophobicity. FEMS Microbiol. Lett., 1980, 9(1), 29-33.
[http://dx.doi.org/10.1111/j.1574-6968.1980.tb05599.x]
[33]
Pradhan, A.K.; Pradhan, N.; Mall, G.; Panda, H.T.; Sukla, L.B.; Panda, P.K.; Mishra, B.K. Application of lipopeptide biosurfactant isolated from a halophile: Bacillus tequilensis CH for inhibition of biofilm. Appl. Biochem. Biotechnol., 2013, 171(6), 1362-1375.
[http://dx.doi.org/10.1007/s12010-013-0428-3 ] [PMID: 23955294]
[34]
Varadavenkatesan, T.; Murty, V.R. Production of a lipopeptide biosurfactant by a novel Bacillus sp. and its applicability to enhanced oil recovery. ISRN microbiology, 2013, 2013(3), 621519.
[35]
Kumar, C.G.; Pombala, S. Process for the preparation of polymeric biosurfactants. US Patent 20, 2015, 150(322), 173.
[36]
Parthipan, P.; Preetham, E.; Machuca, L.L.; Rahman, P.K.; Murugan, K.; Rajasekar, A. Biosurfactant and degradative enzymes mediated crude oil degradation by bacterium Bacillus subtilis A1. Front. Microbiol., 2017, 8, 193.
[http://dx.doi.org/10.3389/fmicb.2017.00193 ] [PMID: 28232826]
[37]
Santos, D.K. Optimization of cultural conditions for biosurfactant production from Candida lipolytica. Biocatal. Agric. Biotechnol., 2014, 3(3), 48-57.
[http://dx.doi.org/10.1016/j.bcab.2014.02.004]
[38]
Anandaraj, B.; Thivakaran, P. Isolation and production of biosurfactant producing organism from oil spilled soil. J Biosci Tech, 2010, 1(3), 120-126.
[39]
Janek, T.; Łukaszewicz, M.; Rezanka, T.; Krasowska, A. Isolation and characterization of two new lipopeptide biosurfactants produced by Pseudomonas fluorescens BD5 isolated from water from the Arctic Archipelago of Svalbard. Bioresour. Technol., 2010, 101(15), 6118-6123.
[http://dx.doi.org/10.1016/j.biortech.2010.02.109 ] [PMID: 20303744]
[40]
Chen, H.; Wang, L.; Su, C.X.; Gong, G.H.; Wang, P.; Yu, Z.L. Isolation and characterization of lipopeptide antibiotics produced by Bacillus subtilis. Lett. Appl. Microbiol., 2008, 47(3), 180-186.
[http://dx.doi.org/10.1111/j.1472-765X.2008.02412.x ] [PMID: 19552782]
[41]
Pecci, Y.; Rivardo, F.; Martinotti, M.G.; Allegrone, G. LC/ESI-MS/MS characterisation of lipopeptide biosurfactants produced by the Bacillus licheniformis V9T14 strain. J. Mass Spectrom., 2010, 45(7), 772-778.
[http://dx.doi.org/10.1002/jms.1767 ] [PMID: 20623484]
[42]
Das, K.; Mukherjee, A.K. Comparison of lipopeptide biosurfactants production by Bacillus subtilis strains in submerged and solid state fermentation systems using a cheap carbon source: some industrial applications of biosurfactants. Process Biochem., 2007, 42(8), 1191-1199.
[http://dx.doi.org/10.1016/j.procbio.2007.05.011]
[43]
Silva, E.J.; Rocha e Silva, N.M.; Rufino, R.D.; Luna, J.M.; Silva, R.O.; Sarubbo, L.A. Characterization of a biosurfactant produced by Pseudomonas cepacia CCT6659 in the presence of industrial wastes and its application in the biodegradation of hydrophobic compounds in soil. Colloids Surf. B Biointerfaces, 2014, 117, 36-41.
[http://dx.doi.org/10.1016/j.colsurfb.2014.02.012 ] [PMID: 24613853]
[44]
Nadh, G. Identification of azo dye degrading Sphingomonas strain EMBS022 and EMBS023 using 16S rRNA gene sequencing. Curr. Bioinform., 2015, 10(5), 599-605.
[http://dx.doi.org/10.2174/1574893610666151008012312]
[45]
Wishard, R.; Jaiswal, M.; Parveda, M.; Amareshwari, P.; Bhadoriya, S.S.; Rathore, P.; Yadav, M.; Nayarisseri, A.; Nair, A.S. Identification and characterization of alkaline protease producing Bacillus firmus species EMBS023 by 16S rRNA gene sequencing. Interdiscip. Sci., 2014, 6(4), 271-278.
[http://dx.doi.org/10.1007/s12539-014-0187-z ] [PMID: 25118655]
[46]
Nayarisseri, A.; Suppahia, A.; Nadh, A.G.; Nair, A.S. Identification and characterization of a pesticide degrading Flavobacterium species EMBS0145 by 16S rRNA gene sequencing. Interdiscip. Sci., 2015, 7(2), 93-99.
[http://dx.doi.org/10.1007/s12539-015-0016-z ] [PMID: 26202942]
[47]
Nayarisseri, A. Impact of Next-Generation Whole-Exome sequencing in molecular diagnostics. Drug Invention Today, 2013, 5(4), 327-334.
[http://dx.doi.org/10.1016/j.dit.2013.07.005]
[48]
Nayarisseri, A.; Singh, P.; Singh, S. Screening, isolation and characterization of biosurfactant-producing Bacillus tequilensis strain ANSKLAB04 from brackish river water. Int. J. Environ. Sci. Technol., 2018, 16(11), 7103-7112.
[http://dx.doi.org/10.1007/s13762-018-2089-9]
[49]
Shah, K.P. Screening, isolation and identification of polygalacturonase producing Bacillus tequilensis strain EMBS083 using 16S rRNA gene sequencing. Eur J Biol Sci, 2013, 5(1), 09-13.
[50]
Chandok, H.; Shah, P.; Akare, U.R.; Hindala, M.; Bhadoriya, S.S.; Ravi, G.V.; Sharma, V.; Bandaru, S.; Rathore, P.; Nayarisseri, A. Screening, isolation and identification of Probiotic producing lactobacillus acidophilus strains EMBS081 & EMBS082 by 16S rRNA gene sequencing. Interdiscip. Sci., 2015, 7(3), 242-248.
[http://dx.doi.org/10.1007/s12539-015-0002-5 ] [PMID: 26199209]
[51]
Phanse, N. Characterization of an industrially important alkalophilic bacterium, Bacillus agaradhaerens strain nandiniphanse5. J. Pharm. Res., 2013, 6(5), 543-550.
[http://dx.doi.org/10.1016/j.jopr.2013.04.035]
[52]
Pyde, A.N. Identification and characterization of foodborne pathogen Listeria monocytogenes strain Pyde1 and Pyde2 using 16S rRNA gene sequencing. J. Pharm. Res., 2013, 6(7), 736-741.
[http://dx.doi.org/10.1016/j.jopr.2013.07.009]
[53]
Krishnan, S.N.; Nayarisseri, A.; Rajamanickam, U. Biodegradation effects of o-cresol by Pseudomonas monteilii SHY on mustard seed germination. Bioinformation, 2018, 14(6), 271-278.
[http://dx.doi.org/10.6026/97320630014271 ] [PMID: 30237672]
[54]
Ron, E.Z.; Rosenberg, E. Biosurfactants and oil bioremediation. Curr. Opin. Biotechnol., 2002, 13(3), 249-252.
[http://dx.doi.org/10.1016/S0958-1669(02)00316-6 ] [PMID: 12180101]
[55]
Amareshwari, P.; Bhatia, M.; Venkatesh, K.; Roja Rani, A.; Ravi, G.V.; Bhakt, P.; Bandaru, S.; Yadav, M.; Nayarisseri, A.; Nair, A.S. Isolation and characterization of a novel chlorpyrifos degrading flavobacterium species EMBS0145 by 16S rRNA gene sequencing. Interdiscip. Sci., 2015, 7(1), 1-6.
[http://dx.doi.org/10.1007/s12539-012-0207-9 ] [PMID: 25248957]
[56]
Bhatia, M.; Girdhar, A.; Tiwari, A.; Nayarisseri, A. Implications of a novel Pseudomonas species on low density polyethylene biodegradation: an in vitro to in silico approach. Springerplus, 2014, 3(1), 497.
[http://dx.doi.org/10.1186/2193-1801-3-497 ] [PMID: 25932357]
[57]
Nayarisseri, A.; Hood, E.A. Advancement in microbial cheminformatics. Curr. Top. Med. Chem., 2018, 18(29), 2459-2461.
[http://dx.doi.org/10.2174/1568026619666181120121528 ] [PMID: 30457050]

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