Login Register Cart 0
Generic placeholder image

Current Drug Delivery

Editor-in-Chief

ISSN (Print): 1567-2018
ISSN (Online): 1875-5704

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Targeted Delivery of Colloidal Silver for MCF-7 Breast Cancer Treatment

Author(s): Shweta Rajawat* and Manzar M. Malik

Volume 17, Issue 7, 2020

Page: [613 - 621] Pages: 9

DOI: 10.2174/1567201817666200508095241

Price: $65

Abstract

Background: Amongst various cancer diseases, breast cancer is frequently diagnosed malignancy in women. Existing treatments are inadequate, painful and toxic. New ways of treatments need to be explored.

Methods: The present work proposes preparation and targeted delivery of a formulation, F-1, for MCF-7 breast cancer treatment. The formulation, colloidal silver (0.76 ppm), was prepared by electrolytic deposition technique and multi surface coatings. Black tea extract (2.25%v/v) was used as a capping agent to tune the morphology of silver nanoparticle and potato extract (6.25%v/v) as a functionalizing agent for targeting MCF-7 breast cancer site.

Results: Characterization results show highly pure spherical silver nanoparticles with an average particle size of 15nm. The shift of peaks in the FTIR spectra of formulation confirms the interaction between nanoparticles and extracts. The UV-visible peak was obtained at 525nm, a typical characteristic of silver nanoparticles. In-vivo anti-cancer study of formulation gave a moderate therapeutic effect in Non-Obese Diabetic Severe Combined Immune Deficiency (NOD-SCID) mice.

Conclusion: It is observed that tumor volumes obtained in the case of Formulation-1 were moderately inhibited from days 5 to 9. However, one of the mice in the Formulation-1 group inhibited tumor volume to 1.52 cc similar to one of the mice of positive control group (Adriamycin 1.42cc).

Keywords: MCF-7 breast cancer, multi surface coatings, black tea leaves extract, potato extract, silver nanoparticles, Adriamycin, targeted delivery.

« Previous Next »
Graphical Abstract

[1]
Gupta, E.; Kaushik, S.; Purwar, S.; Sharma, R.; Balapure, A.K.; Sundaram, S. Anticancer potential of steviol in MCF-7 human breast cancer cells. Pharmacogn. Mag., 2017, 13(51), 345-350.
[http://dx.doi.org/10.4103/pm.pm_29_17] [PMID: 28839355]
[2]
Wang, C.; Dong, S.; Zhang, L.; Zhao, Y.; Huang, L.; Gong, X.; Wang, H.; Shang, D. Cell surface binding, uptaking and anticancer activity of L-K6, a lysine/leucine-rich peptide, on human breast cancer MCF-7 cells. Sci. Rep., 2017, 7(1), 8293.
[http://dx.doi.org/10.1038/s41598-017-08963-2] [PMID: 28811617]
[3]
Desingu, K.; Natarajan, D. Anti- cancer potential of leaf and leaf-derived callus extracts of Aerva javanica against MCF-7 breast cancer cell line. J. Cancer Res. Ther., 2018, 14(2), 321-327.
[4]
Gamucci, O.; Bertero, A.; Gagliardi, M.; Bardi, G. biomedical nanoparticles: overview of their surface immune-compatibility. Coatings, 2014, 4, 139-159.
[http://dx.doi.org/10.3390/coatings4010139]
[5]
Pace Pereira Lima, G.; Vianello, F.; Renata Corrêa, C.; Arnoux da Silva Campos, R.; Galhardo-Borguini, M. Polyphenols in fruits and vegetables and its effect on human health. Food Nutr. Sci., 2014, 5(11), 1065-1082.
[6]
Kuhnert, N. Unraveling the structure of the black tea thearubigins. Arch. Biochem. Biophys., 2010, 501(1), 37-51.
[http://dx.doi.org/10.1016/j.abb.2010.04.013] [PMID: 20430006]
[7]
Farr, T.D.; Lai, C.H.; Grünstein, D.; Orts-Gil, G.; Wang, C.C.; Boehm-Sturm, P.; Seeberger, P.H.; Harms, C. Imaging early endothelial inflammation following stroke by core shell silica superparamagnetic glyconanoparticles that target selectin. Nano Lett., 2014, 14(4), 2130-2134.
[http://dx.doi.org/10.1021/nl500388h] [PMID: 24564342]
[8]
Mammen, M.; Choi, S-K.; Whitesides, G.M. Polyvalent interactions in biological systems: implications for design and use of multivalent ligands and inhibitors. Angew. Chem. Int. Ed. Engl., 1998, 37(20), 2754-2794.
[http://dx.doi.org/10.1002/(SICI)1521-3773(19981102)37:20<2754:AID-ANIE2754>3.0.CO;2-3] [PMID: 29711117]
[9]
Rajawat, Shweta.; Kurchania, Rajnish. Katherukamen, Rajukumar.; Pitale, Shreyas; Saha, Sonali; Qureshi, M.S Study of anti-cancer properties of green silver nanoparticles against MCF-7 breast cancer cell lines. Green Process Synth., 2016, 5, 173-181.
[10]
Khah, D.J.V. Dowlat Sara; Rouhollahi, A.; Pourmortazavi, S.M.; Shamsipur M. High current density chronopotentiometric electrosynthesis and SEM characterization of hexanethiol-monolayer-protected silver planar nanotriangles (Ag@C6SH). J. Nanomat., 2016, 12, e5042670.
[11]
Laroo, H. Colloidal nano silver-its production method, properties, standards and its bio-efficac an inorganic antibiotic. Phys. Chem. Biophys., 2013, 3, 130.
[12]
Meng, Y. A sustainable approach to fabricating Ag nanoparticles/PVA hybrid nanofiber and its catalytic activity. Nanomaterials (Basel), 2015, 5(2), 1124-1135.
[http://dx.doi.org/10.3390/nano5021124] [PMID: 28347055]
[13]
Priyadharshini, R.I.; Prasannaraj, G.; Geetha, N.; Venkatachalam, P. Microwave-mediated extracellular synthesis of metallic silver and zinc oxide nanoparticles using macro-algae (Gracilaria edulis) extracts and its anticancer activity against human PC3 cell lines. Appl. Biochem. Biotechnol., 2014, 174(8), 2777-2790.
[http://dx.doi.org/10.1007/s12010-014-1225-3] [PMID: 25380639]
[14]
Roy, K.; Sarkar, C.K.; Ghosh, C.K. Green snthesis of silver nanoparticles using fruit extract of Malus domestica and study of its antimicrobial activity. Dig. J. Nanomater. Biostruct., 2014, 9, 1137-1147.
[15]
Manish, D.; Seema, B.; Kushwaha, B.S. Green synthesis of nanosilver particles from extract of Eucalyptus Hybrida (Safeda) leaf digest. J. Nanomat. Biostruct., 2009, 4(3), 537-543.
[16]
Peter, I. Kattan, “Ratio of Surface Area to Volume in Nanotechnology and Nanoscience”, Basic Nano mechanics Series; Petra Books, 2011.
[17]
Buazar, F.; Bavi, M.; Kroushawi, F.; Halvani, M.; Khaledi-Nasab, A.; Hossieni, S.A. Potato extract as reducing agent and stabilizer in a facile green one-step synthesis of ZnO nanoparticles. J. Exp. Nanosci., 2016, 11, 3.
[http://dx.doi.org/10.1080/17458080.2015.1039610]
[18]
Vanaja, M.; Paulkumar, K.; Baburaja, M.; Rajeshkumar, S.; Gnanajobitha, G.; Malarkodi, C.; Sivakavinesan, M.; Annadurai, G. Degradation of methylene blue using biologically synthesized silver nanoparticles. Bioinorg. Chem. ApplI., 2014, 8, e742346.
[http://dx.doi.org/10.1155/2014/742346]
[19]
Raubenheimer, H.G.; Dobrzanska, L. Interaction between Cu and Ag free ions and central metals in complexes with XHn units (X = B, Si, N, O, C, Al, Zn, Mg; n = 1, 2). Coord. Chem. Rev., 2020, 402, 213052.
[http://dx.doi.org/10.1016/j.ccr.2019.213052]
[20]
Koths, K.; Taylor, E.; Halenbeck, R.; Casipit, C.; Wang, A. Cloning and characterization of a human Mac-2-binding protein, a new member of the superfamily defined by the macrophage scavenger receptor cysteine-rich domain. J. Biol. Chem., 1993, 268(19), 14245-14249.
[PMID: 8390986]
[21]
Resnick, D.; Pearson, A.; Krieger, M. The SRCR superfamily: a family reminiscent of the Ig superfamily. Trends Biochem. Sci., 1994, 19(1), 5-8.
[http://dx.doi.org/10.1016/0968-0004(94)90165-1] [PMID: 8140623]
[22]
Ullrich, A.; Sures, I.; D’Egidio, M.; Jallal, B.; Powell, T.J.; Herbst, R.; Dreps, A.; Azam, M.; Rubinstein, M.; Natoli, C. The secreted tumor-associated antigen 90K is a potent immune stimulator. J. Biol. Chem., 1994, 269(28), 18401-18407.
[PMID: 8034587]
[23]
Nonaka, M.; Ma, B.Y.; Imaeda, H.; Kawabe, K.; Kawasaki, N.; Hodohara, K.; Kawasaki, N.; Andoh, A.; Fujiyama, Y.; Kawasaki, T. Dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN) recognizes a novel ligand, Mac-2-binding protein, characteristically expressed on human colorectal carcinomas. J. Biol. Chem., 2011, 286(25), 22403-22413.
[http://dx.doi.org/10.1074/jbc.M110.215301] [PMID: 21515679]
[24]
D’Ostilio, N.; Sabatino, G.; Natoli, C.; Ullrich, A.; Iacobelli, S. 90K (Mac-2 BP) in human milk. Clin. Exp. Immunol., 1996, 104(3), 543-546.
[http://dx.doi.org/10.1046/j.1365-2249.1996.40745.x] [PMID: 9099942]
[25]
White, M.J.V.; Roife, D.; Gomer, R.H. Galectin-3 binding protein secreted by breast cancer cells inhibits monocyte-derived fibrocyte differentiation. J. Immunol., 2015, 195(4), 1858-1867.
[http://dx.doi.org/10.4049/jimmunol.1500365] [PMID: 26136428]
[26]
Hirabayashi, J.; Kasai, K. The family of metazoan metal-independent beta-galactoside-binding lectins: structure, function and molecular evolution. Glycobiology, 1993, 3(4), 297-304.
[http://dx.doi.org/10.1093/glycob/3.4.297] [PMID: 8400545]
[27]
GeneCards 2019.https://www.genecards.org/cgi-bin/carddisp. pl? gene=ALDH1A1
[28]
Kong, Y.; Lyu, N.; Wu, J.; Tang, H.; Xie, X.; Yang, L.; Li, X.; Wei, W.; Xie, X. Breast cancer stem cell markers CD44 and ALDH1A1 in serum: distribution and prognostic value in patients with primary breast cancer. J. Cancer, 2018, 9(20), 3728-3735.
[http://dx.doi.org/10.7150/jca.28032] [PMID: 30405844]
[29]
Onitilo, A.A.; Engel, J.M.; Greenlee, R.T.; Mukesh, B.N. Breast cancer subtypes based on ER/PR and Her2 expression: comparison of clinicopathologic features and survival. Clin. Med. Res., 2009, 7(1-2), 4-13.
[http://dx.doi.org/10.3121/cmr.2008.825] [PMID: 19574486]

Rights & Permissions Print Cite
Article Metrics
12
2
© 2024 Bentham Science Publishers | Privacy Policy