Abstract
Background: According to the World Health Organization (WHO), two-fifths of the world population is at risk of infection by DENV. There are no safe and effective vaccines established. Sulfated glycosaminoglycans such as heparin, used as anticoagulants, inhibit the initial step of dengue viral replication. Recently, an isolated heparin analogue Goniopsis cruentata (cCTH) has presented a low anticoagulant effect with reduced bleeding risk.
Methods: The antiviral activity of cCTH and heparin compounds against DENV-2 in Vero cell culture was determined by quantitative RT-PCR (qRT-PCR) and titration. For this, four trials were carried out: treatment of the cells for 2 h before viral inoculation, concomitant viral inoculation treatment, treatment after viral inoculation and virucidal assay. Subsequently, the culture supernatants were collected for periods of 24, 48 and 72 h.
Results: Our results demonstrated that cCTH and heparin showed antiviral activity against DENV-2.
Conclusion: These data suggest that both compounds prevented viral replication in cultured Vero cells.
Keywords: Vero cells, DENV-2, Heparin, Goniopsis cruentata, antiviral, qRT-PCR, titration.
Graphical Abstract
Anti-Infective Agents
Title:The Crab Heparin-like Compound Exhibits a Strong Inhibitory Effect on Infections by Dengue Virus-2
Volume: 19 Issue: 1
Author(s): Marlyanne M.C.S. de Almeida, Renato F. de A. Júnior, Ana A. de Aquino, Lívia de Lourdes Pinto, Paula R.L. Machado, Suely F. Chavante, Kleber J.S. Farias*Giulianna P.V. de Andrade
Affiliation:
- Center of Education and Health, Federal University of Campina Grande, 58175-000, Cuite, PB,Brazil
Keywords: Vero cells, DENV-2, Heparin, Goniopsis cruentata, antiviral, qRT-PCR, titration.
Abstract:
Background: According to the World Health Organization (WHO), two-fifths of the world population is at risk of infection by DENV. There are no safe and effective vaccines established. Sulfated glycosaminoglycans such as heparin, used as anticoagulants, inhibit the initial step of dengue viral replication. Recently, an isolated heparin analogue Goniopsis cruentata (cCTH) has presented a low anticoagulant effect with reduced bleeding risk.
Methods: The antiviral activity of cCTH and heparin compounds against DENV-2 in Vero cell culture was determined by quantitative RT-PCR (qRT-PCR) and titration. For this, four trials were carried out: treatment of the cells for 2 h before viral inoculation, concomitant viral inoculation treatment, treatment after viral inoculation and virucidal assay. Subsequently, the culture supernatants were collected for periods of 24, 48 and 72 h.
Results: Our results demonstrated that cCTH and heparin showed antiviral activity against DENV-2.
Conclusion: These data suggest that both compounds prevented viral replication in cultured Vero cells.
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Cite this article as:
de Almeida M.C.S. Marlyanne , de A. Júnior F. Renato , de Aquino A. Ana , de Lourdes Pinto Lívia , Machado R.L. Paula , Chavante F. Suely , Farias J.S. Kleber *, de Andrade P.V. Giulianna , The Crab Heparin-like Compound Exhibits a Strong Inhibitory Effect on Infections by Dengue Virus-2, Anti-Infective Agents 2021; 19 (1) . https://dx.doi.org/10.2174/2211352518999200429105342
DOI https://dx.doi.org/10.2174/2211352518999200429105342 |
Print ISSN 2211-3525 |
Publisher Name Bentham Science Publisher |
Online ISSN 2211-3533 |
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