Login Register Cart 0
Generic placeholder image

Current Bioactive Compounds

Editor-in-Chief

ISSN (Print): 1573-4072
ISSN (Online): 1875-6646

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Chemical Composition and Bioactivity of Essential Oil and Extracts of Salvia limbata C.A. Mey (Lamiaceae)

Author(s): Behnam Mahdavi*, Somaye Ghezi and Behroz Maleki

Volume 16, Issue 9, 2020

Page: [1299 - 1305] Pages: 7

DOI: 10.2174/1573407216666200218111906

Price: $65

Abstract

Background: This research tried to evaluate the chemical composition of the essential oil of Salvia limbata, and also bioactivity of essential oil and the plant extract. S. limbata is an aromatic plant with medicinal uses, such as analgesic, anti-inflammatory activities.

Methods: The essential oils of the flowers of Salvia limbata were obtained using a Clevenger-type apparatus, and its chemical composition was identified by chromatography methods. The study was also conducted to evaluate antioxidant and antibacterial activities of the plant extracts. For this purpose, the leaves and flowers of S. limbata were extracted using methanol and then fractioned by n-hexane and Ethyl Acetate (EtOAc). Total Phenolic Content (TPC), Total Flavonoid Content (TFC), Radical Scavenging Activity (RSA) and the Disc-Diffusion (DD) assay were run to study the bioactivity of the plant extracts.

Results: The oil was dominated by the oxygenated hydrocarbon of 5-methylene-2,3,4,4-tetramethylcyclopent- 2-enone (37.1%), oxygenated sesquiterpene of occidol (22.8%), and sesquiterpene hydrocarbon of β-bourbonene (12.1%). The EtOAc extract of the flowers showed the TPC with 66.2±0.62 mg GAE/g (mg of gallic acid equivalents per gram of extract) and RSA with IC50 of 17.96±0.54 μg/mL. Although the EtOAc extract of the leaves exhibited the highest TFC with 61.96±4.73 mg RuE/g (mg of rutin equivalents per gram of extract). The extracts inhibited the growth of Escherichia coli, Citrobacter frurdi, Pseudomonas aeruginosa, and Streptococcus pneumonia. The extracts were inactive against Staphylococcus coagulase, Enterobacter aerogenes, Acinetobacter baumannii, Serratia marcescens, and Klebsiella pneumoni.

Conclusion: According to the results, the plant extracts were potent agant in antioxidant activity.

Keywords: Salvia limbata, Lamiaceae, occidol, β-bourbonene, antioxidant activity, antibacterial activity.

Graphical Abstract

[1]
Saeidnia, S.; Gohari, A.; Malmir, M.; Moradi-Afrapoli, F.; Ajani, Y. Tryptophan and sterols from Salvia limbata. J. Med. Plant., 2011, 1(37), 41-47.
[2]
Sajjadi, S.E.; Shahpiri, Z. Chemical composition of the essential oil of Salvia limbata C.A. Mey. Daru, 2004, 12(3), 94-97.
[3]
Rajabi, Z.; Ebrahimi, M.; Farajpour, M.; Mirza, M.; Ramshini, H. Compositions and yield variation of essential oils among and within nine Salvia species from various areas of Iran. Ind. Crops Prod., 2014, 61, 233-239.
[http://dx.doi.org/10.1016/j.indcrop.2014.06.038]
[4]
Karami, M.; Hossini, E.; Majd, N.S.; Ebrahimzadeh, M.A.; Alemy, S. Salvia limbata: Botanical, chemical, pharmacological and therapeutic effecte. J. Clin. Excell., 2015, 3(2), 1-14.
[5]
Hosseinzadeh, H.; Sadeghnia, H.R.; Imenshahidi, M.; Fazly Bazzaz, B.S. Review of the pharmacological and toxicological effects of Salvia leriifolia. Iran. J. Basic Med. Sci., 2009, 12(1), 1-8.
[6]
Savelev, S.U.; Okello, E.J.; Perry, E.K. Butyryl- and acetyl-cholinesterase inhibitory activities in essential oils of Salvia species and their constituents. Phytother. Res., 2004, 18(4), 315-324.
[http://dx.doi.org/10.1002/ptr.1451] [PMID: 15162368]
[7]
Guaouguaou, F.E.; Taghzouti, K.; Oukabli, M.; Es-Safi, N.E. The effect of Salvia verbenaca extracts for healing of second-degree burn wounds in rats. Curr. Bioact. Compd., 2018, 14(4), 419-427.
[http://dx.doi.org/10.2174/1573407213666170621074336]
[8]
Farhoosh, R.; Purazrang, H.; Khodaparast, M.; Rahimizadeh, M.; Seyedi, S. Extraction and separation of antioxidative compounds from salvia leriifolia leaves. J. Agric. Sci. Technol., 2004, 6(1-2), 57-62.
[9]
Loizzo, M.R.; Menichini, F.; Tundis, R.; Bonesi, M.; Nadjafi, F.; Saab, A.M.; Frega, N.G.; Menichini, F. Comparative chemical composition and antiproliferative activity of aerial parts of Salvia leriifolia Benth. and Salvia acetabulosa L. essential oils against human tumor cell in vitro models. J. Med. Food, 2010, 13(1), 62-69.
[http://dx.doi.org/10.1089/jmf.2009.0060] [PMID: 20136437]
[10]
Adams, R.P. Identification of essential oils components by gas chromatography/quaderupole mass spectroscopy; Allured: Illinois, 2007.
[11]
NIST D. Nist data base national institute of standards and technology; Department of Commerce: Gaithersburg, MD, U.S., 2005.
[12]
Sarker, S.D.; Latif, Z.; Gray, A.I. Natural products isolation, 2nd ed; Springer Science & Business Media, 2005.
[http://dx.doi.org/10.1385/1592599559]
[13]
Mahdavi, B.; Yaacob, W.A.; Laily, B.D.; Jahangirian, H. Antioxidant activity of consecutive extracts of the base, stem and leaves of Etlingera brevilabrum. Asian J. Chem., 2013, 25(7), 3937-3941.
[http://dx.doi.org/10.14233/ajchem.2013.13851]
[14]
Karamian, R.; Ghasemlou, F. Screening of total phenol and flavonoid content, antioxidant and antibacterial activities of the methanolic ex-tracts of three silene species from Iran. Int. J. Agric. Crop Sci., 2013, 5(3), 305.
[15]
Abadi, Z.H.M.; Mahdavi, B.; Rezaei-Seresht, E. Contents of aerial parts of Salvia leriifolia benth. J. Chem. Health Risks, 2016, 6(3), 1-10.
[16]
Mahdavi, B.; Yaacob, W.A.; Laily, B.D.; Nazlina, I. Antimicrobial activity of consecutive extracts of Etlingera brevilabrum. Sains Malays., 2012, 41(10), 1233-1237.
[17]
Chan, E.; Lim, Y.; Omar, M. Antioxidant and antibacterial activity of leaves of etlingera species (zingiberaceae) in peninsular malaysia. Food Chem., 2007, 104(4), 1586-1593.
[http://dx.doi.org/10.1016/j.foodchem.2007.03.023]
[18]
Alimpić, A.; Pljevljakušić, D.; Šavikin, K.; Knežević, A.; Ćurčić, M.; Veličković, D. Composition and biological effects of Salvia ringens (lamiaceae) essential oil and extracts. Ind. Crops Prod., 2015, 76, 702-709.
[http://dx.doi.org/10.1016/j.indcrop.2015.07.053]
[19]
Bahadori, M.B.; Valizadeh, H.; Asghari, B.; Dinparast, L.; Farimani, M.M.; Bahadori, S. Chemical composition and antimicrobial, cytotoxicity, antioxidant and enzyme inhibitory activities of Salvia spinosa l. J. Funct. Foods, 2015, 18, 727-736.
[http://dx.doi.org/10.1016/j.jff.2015.09.011]
[20]
Mohammadhosseini, M. Chemical composition of the essential oils and volatile fractions from flowers, stems and roots of Salvia multicaulis vahl. By using MAHD, SFME and HS-SPME methods. J. Essent. Oil Bear. Pl., 2015, 18(6), 1360-1371.
[http://dx.doi.org/10.1080/0972060X.2015.1024447]
[21]
Sepahvand, R.; Delfan, B.; Ghanbarzadeh, S.; Rashidipour, M.; Veiskarami, G.H.; Ghasemian-Yadegari, J. Chemical composition, antioxidant activity and antibacterial effect of essential oil of the aerial parts of Salvia sclareoides. Asian Pac. J. Trop. Med., 2014, 7, 491-496.
[22]
Tel, G.; Oztürk, M.; Duru, M.E.; Harmandar, M.; Topçu, G. Chemical composition of the essential oil and hexane extract of Salvia chionan-tha and their antioxidant and anticholinesterase activities. Food Chem. Toxicol., 2010, 48(11), 3189-3193.
[http://dx.doi.org/10.1016/j.fct.2010.08.020] [PMID: 20732375]
[23]
Valifard, M.; Mohsenzadeh, S.; Kholdebarin, B.; Rowshan, V. Effects of salt stress on volatile compounds, total phenolic content and antioxidant activities of Salvia mirzayanii. S. Afr. J. Bot., 2014, 93, 92-97.
[http://dx.doi.org/10.1016/j.sajb.2014.04.002]
[24]
Öğütçü, H.; Sökmen, A.; Sökmen, M.; Polissiou, M.; Serkedjieva, J.; Daferera, D. Bioactivities of the various extracts and essential oils of Salvia limbata C.A. Mey. and Salvia sclarea l. Turk. J. Biol., 2008, 32(3), 181-192.
[25]
Rustaiyan, A.; Akhgar, M.R.; Masoudi, S.; Nematollahi, F. Chemical composition of essential oils of three salvia species growing wild in Iran: Salvia rhytidea benth., S. Limbata ca Mey. and S. Palaestina benth. J. Essent. Oil Res., 2005, 17(5), 522-524.
[http://dx.doi.org/10.1080/10412905.2005.9698982]
[26]
Shakeri, A.; Sharifi, M.J.; Fazly Bazzaz, B.S.; Emami, A.; Soheili, V.; Sahebkar, A.H.; Asili, J. bioautography detection of antimicrobial compounds from the essential oil of Salvia pachystachys. Curr. Bioact. Compd., 2018, 14(1), 80-85.
[http://dx.doi.org/10.2174/1573407212666161014132503]
[27]
Sakai, A.; Yoshimura, H. Monoterpenes of Salvia leucophylla. Curr. Bioact. Compd., 2012, 8(1), 90-100.
[http://dx.doi.org/10.2174/157340712799828205] [PMID: 22754426]
[28]
Firouznia, A.; Rustaiyana, A.; Masoudi, S.; Rahimizade, M.; Bigdeli, M.; Tabatabaei-Anaraki, M. Volatile constituents of Salvia limbata, stachys turcomanica, Scutellaria litwinowii and Hymenocrater elegans four lamiaceae herbs from Iran. J. Essent. Oil Bear. Pl., 2009, 12(4), 482-489.
[http://dx.doi.org/10.1080/0972060X.2009.10643748]
[29]
Salehi, P.; Sonboli, A.; Dayeni, M.; Eftekhar, F.; Yousefzadi, M. Chemical composition of essential oils of Salvia limbata from two different regions in Iran and their biological activities. Chem. Nat. Compd., 2008, 44(1), 102-105.
[http://dx.doi.org/10.1007/s10600-008-0030-z]
[30]
Aissi, O.; Boussaid, M.; Messaoud, C. Essential oil composition in natural populations of Pistacia lentiscus l. From Tunisia: Effect of ecological factors and incidence on antioxidant and antiacetylcholinesterase activities. Ind. Crops Prod., 2016, 91, 56-65.
[http://dx.doi.org/10.1016/j.indcrop.2016.06.025]
[31]
Teles, S.; Pereira, J.A.; Santos, C.H.B.; Menezes, R.V.; Malheiro, R.; Lucchese, A.M. Geographical origin and drying methodology may affect the essential oil of Lippia alba (mill)n.E. Brown. Ind. Crop. Prod; , 2012; 31, pp. (1)247-5.
[32]
Formisano, C.; Delfine, S.; Oliviero, F.; Tenore, G.C.; Rigano, D.; Senatore, F. Correlation among environmental factors, chemical com-position and antioxidative properties of essential oil and extracts of chamomile (Matricaria chamomilla l.) collected in molise (south-central italy). Ind. Crops Prod., 2015, 63, 256-263.
[http://dx.doi.org/10.1016/j.indcrop.2014.09.042]
[33]
Noroozloo, Y.; Mirjalili, M.; Nazeri, V.; Araghi, A. Evaluation of some ecological factors, morphological traits and essential oil productivity of Stachys lavandulifolia vahl. In four provinces of Iran. Iran. J. Med. Aroma. Pl., 2015, 30(6), 985-998.
[34]
Salehi, S.P.; Mirza, M.; Calagari, M.; Adams, R.P. Effects drying and harvest season on the essential oil composition from foliage and berries of Juniperus excelsa. Ind. Crops Prod., 2010, 32(2), 83-87.
[http://dx.doi.org/10.1016/j.indcrop.2010.03.003]
[35]
Yousefi, M.; Nazeri, V.; Mirza, M. Effects of environmental conditions on the quantity and quality of Salvia leriifolia benth. Essential oil. Iran. J. Med. Aroma. Pl., 2015, 30(6), 861-878.
[36]
Farhat, M.B.; Landoulsi, A.; Chaouch-Hamada, R.; Sotomayor, J.A.; Jordán, M.J. Characterization and quantification of phenolic compounds and antioxidant properties of Salvia species growing in different habitats. Ind. Crops Prod., 2013, 49, 904-914.
[http://dx.doi.org/10.1016/j.indcrop.2013.06.047]
[37]
Orhan, I.E.; Senol, F.S.; Ercetin, T.; Kahraman, A.; Celep, F.; Akaydin, G. Assessment of anticholinesterase and antioxidant properties of selected sage (salvia) species with their total phenol and flavonoid contents. Ind. Crops Prod., 2013, 41, 21-30.
[http://dx.doi.org/10.1016/j.indcrop.2012.04.002]
[38]
Dinçer, C.; Tontul, I.; Cam, I.B. Phenolic composition and antioxidant activity of Salvia tomentosa miller: Effects of cultivation, harvesting year, and storage. Turk. J. Agric. For., 2013, 37(5), 561-567.
[http://dx.doi.org/10.3906/tar-1211-72]
[39]
Roby, M.H.H.; Sarhan, M.A.; Selim, K.A-H.; Khalel, K.I. Evaluation of antioxidant activity, total phenols and phenolic compounds in thyme (Thymus vulgaris l.), sage (Salvia officinalis l.), and marjoram (Origanum majorana l.) extracts. Ind. Crops Prod., 2013, 43, 827-831.
[http://dx.doi.org/10.1016/j.indcrop.2012.08.029]
[40]
Nickavar, B.; Kamalinejad, M.; Izadpanah, H. In vitro free radical scavenging activity of five Salvia species. Pak. J. Pharm. Sci., 2007, 20(4), 291-294.
[PMID: 17604251]
[41]
Bahadori, M.B.; Valizadeh, H.; Asghari, B.; Dinparast, L.; Bahadori, S.; Moridi Farimani, M. Biological activities of Salvia santolinifolia Boiss. A multifunctional medicinal plant. Curr. Bioact. Compd., 2016, 12(4), 297-305.
[http://dx.doi.org/10.2174/1573407212666160426161112]
[42]
Ebrahimabadi, A.H.; Mazoochi, A.; Kashi, F.J.; Djafari-Bidgoli, Z.; Batooli, H. Essential oil composition and antioxidant and antimicrobial properties of the aerial parts of Salvia eremophila Boiss. from Iran. Food Chem. Toxicol., 2010, 48(5), 1371-1376.
[http://dx.doi.org/10.1016/j.fct.2010.03.003] [PMID: 20211675]
[43]
Firuzi, O.; Miri, R.; Asadollahi, M.; Eslami, S.; Jassbi, A.R. Cytotoxic, antioxidant and antimicrobial activities and phenolic contents of eleven Salvia species from Iran. Iran. J. Pharm. Res., 2013, 12(4), 801-810.
[PMID: 24523760]

Rights & Permissions Print Cite
Article Metrics
7
© 2024 Bentham Science Publishers | Privacy Policy