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Review Article

利用可再生原料微生物合成药物和营养品的策略

卷 27, 期 28, 2020

页: [4613 - 4621] 页: 9

弟呕挨: 10.2174/0929867327666200212121047

价格: $65

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摘要

背景:丰富和可再生的生物材料是各种化学品可持续生产的理想基质，包括天然产品(如药品和营养品)。几十年来，研究人员一直专注于如何设计微生物和开发有效的发酵过程，以从生物材料中过量生产这些分子。尽管取得了许多实验室成果，但要将其中一些转化为成功的工业应用仍是一个挑战。 结果:在此，我们综述了近年来在天然产物生产的代谢工程策略和应用方面的进展。模块化工程方法，如多维启发式过程显着提高效率的优化漫长和复杂的生物合成途径。动态途径调控实现了自主调节，可对代谢碳通量进行重定向，避免有毒中间代谢物的积累。通过引入不同物种之间的竞争或合作，微生物的共同培养促进了天然产物的识别和过度生产。外排工程用于降低产品毒性或克服储存限制，从而提高产品的效价和生产率。 结论:毫无疑问，在自然产物的生物合成方面，许多创新的方法和策略正在逐步催化从实验室到工业的转变。有时，有必要结合两种或两种以上的战略，以获得附加或协同效益。因此，我们可以预见微生物利用可再生生物材料合成药物和营养药品的光明前景。

关键词: 天然产物、次生代谢物、代谢工程、合成生物学、模块化工程、动态调节、微生物共培养、外排工程。

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