Login Register Cart 0
General Research Article

两种转录因子组合对人表皮黑素细胞诱导的多能干细胞的表征。

卷 19, 期 6, 2019

页: [395 - 403] 页: 9

弟呕挨: 10.2174/1566523220666200211105228

价格: $65

摘要

目的：为了更有效地产生诱导性多能干细胞（iPSC），至关重要的是要确定易于进入且可能需要较少因子转化为iPSC的体细胞。 方法：用含有3种转录因子（OCT-4，KLF-4和c-MYC，3F）或4种转录因子（OCT-4，KLF-4，c-MYC和SOX-2）的慢病毒载体转导人表皮黑素细胞， 4F）。一旦克隆形成，就进行与干细胞多能性有关的测定，包括碱性磷酸酶染色，DNA甲基化水平，干细胞标志物的表达和超微结构分析。然后诱导获得的iPSCs在体外分化为代表三个胚胎层的细胞。 结果：在用3F或4F转导表皮黑素细胞后的7天，形成了碱性磷酸酶染色呈阳性的克隆。用针对OCT-4和SOX-2的抗体进行的荧光染色呈强阳性，并且细胞显示出高的细胞核质比和活跃的核运动。通过超微结构分析在细胞质中未发现黑素体。克隆细胞与其亲代细胞之间的DNA甲基化水平存在明显差异。但是，在3F或4F转染的克隆细胞之间没有显着差异。同时，iPSCs在体外成功分化为三个胚层细胞。 结论：人类表皮黑素细胞不需要异位SOX-2表达即可转化为iPSC，并且可以作为获得具有较少遗传成分的患者特异性iPSC的替代来源。

关键词: 表皮，黑素细胞，多能，重编程，iPSCS，转录。

« Previous Next »
图形摘要

[1]
Malik N, Rao MS. A review of the methods for human iPSC derivation. Methods Mol Biol 2013; 997: 23-33.
[http://dx.doi.org/10.1007/978-1-62703-348-0_3] [PMID: 23546745]
[2]
Kawakami T, Okano T, Takeuchi S, et al. Approach for the derivation of melanocytes from induced pluripotent stem cells. J Invest Dermatol 2018; 138(1): 150-8.
[http://dx.doi.org/10.1016/j.jid.2017.07.849] [PMID: 28887108]
[3]
Mortada I, Bilani N. Advances in the production and application of induced pluripotent stem cells. Curr Stem Cell Res Ther 2017; 12(8): 637-43.
[http://dx.doi.org/10.2174/1574888X12666170927115317] [PMID: 28969537]
[4]
Li J, Yin X, Luan Q. Comparative study of periodontal differentiation propensity of induced pluripotent stem cells from different tissue origins. J Periodontol 2018; 89(10): 1230-40.
[http://dx.doi.org/10.1002/JPER.18-0033] [PMID: 30039603]
[5]
Nishihara K, Shiga T, Nakamura E, et al. Induced pluripotent stem cells reprogrammed with three inhibitors show accelerated differentiation potentials with high levels of 2-cell stage marker expression. Stem Cell Reports 2019; 12(2): 305-18.
[http://dx.doi.org/10.1016/j.stemcr.2018.12.018] [PMID: 30713040]
[6]
Wu R, Du D, Bo Y, Zhang M, Zhang L, Yan Y. Hsp90α promotes the migration of iPSCs-derived keratinocyte to accelerate deep second-degree burn wound healing in mice. Biochem Biophys Res Commun 2019; 520(1): 145-51.
[http://dx.doi.org/10.1016/j.bbrc.2019.09.120] [PMID: 31582220]
[7]
Sugiyama-Nakagiri Y, Fujimura T, Moriwaki S. Induction of skin-derived precursor cells from human induced pluripotent stem cells. PLoS One 2016; 11(12): e0168451
[http://dx.doi.org/10.1371/journal.pone.0168451] [PMID: 27992514]
[8]
Utikal J, Maherali N, Kulalert W, Hochedlinger K. Sox2 is dispensable for the reprogramming of melanocytes and melanoma cells into induced pluripotent stem cells. J Cell Sci 2009; 122(Pt 19): 3502-10.
[http://dx.doi.org/10.1242/jcs.054783] [PMID: 19723802]
[9]
Popovic A, Wiggins T, Davids LM. Differential susceptibility of primary cultured human skin cells to hypericin PDT in an in vitro model. J Photochem Photobiol B 2015; 149: 249-56.
[http://dx.doi.org/10.1016/j.jphotobiol.2015.06.009] [PMID: 26114219]
[10]
Adema GJ, de Boer AJ, van ’t Hullenaar R, et al. Melanocyte lineage-specific antigens recognized by monoclonal antibodies NKI-beteb, HMB-50, and HMB-45 are encoded by a single cDNA. Am J Pathol 1993; 143(6): 1579-85.
[PMID: 7504884]
[11]
Raab S, Klingenstein M, Liebau S, Linta L. A comparative view on human somatic cell sources for iPSC generation. Stem Cells Int 2014; 2014: 768391
[http://dx.doi.org/10.1155/2014/768391] [PMID: 25431601]
[12]
Buganim Y, Markoulaki S, van Wietmarschen N, et al. The developmental potential of iPSCs is greatly influenced by reprogramming factor selection. Cell Stem Cell 2014; 15(3): 295-309.
[http://dx.doi.org/10.1016/j.stem.2014.07.003] [PMID: 25192464]
[13]
Streckfuss-Bömeke K, Wolf F, Azizian A, et al. Comparative study of human-induced pluripotent stem cells derived from bone marrow cells, hair keratinocytes, and skin fibroblasts. Eur Heart J 2013; 34(33): 2618-29.
[http://dx.doi.org/10.1093/eurheartj/ehs203] [PMID: 22798560]
[14]
Liu Q, Zhang RZ, Li D, et al. Muse cells, a new type of pluripotent stem cell derived from human fibroblasts. Cell Reprogram 2016; 18(2): 67-77.
[http://dx.doi.org/10.1089/cell.2015.0085] [PMID: 27055628]
[15]
Begemann I, Galic M. Correlative light electron microscopy: Connecting synaptic structure and function. Front Synaptic Neurosci 2016; 8: 28.
[http://dx.doi.org/10.3389/fnsyn.2016.00028] [PMID: 27601992]
[16]
Badja C, Maleeva G, El-Yazidi C, et al. Efficient and cost-effective generation of mature neurons from human induced pluripotent stem cells. Stem Cells Transl Med 2014; 3(12): 1467-72.
[http://dx.doi.org/10.5966/sctm.2014-0024] [PMID: 25355730]
[17]
Muchkaeva IA, Dashinimaev EB, Artyuhov AS, et al. Generation of iPS cells from human hair follice dermal papilla cells. Acta Naturae 2014; 6(1): 45-53.
[http://dx.doi.org/10.32607/20758251-2014-6-1-45-53] [PMID: 24772326]
[18]
Novak D, Weina K, Utikal J. From skin to other cell types of the body. J Dtsch Dermatol Ges 2014; 12(9): 789-92.
[http://dx.doi.org/10.1111/ddg.12403] [PMID: 25087921]
[19]
Khazaei M, Ahuja CS, Fehlings MG. Induced pluripotent stem cells for traumatic spinal cord injury. Front Cell Dev Biol 2017; 4: 152.
[http://dx.doi.org/10.3389/fcell.2016.00152] [PMID: 28154814]
[20]
Abou-Saleh H, Zouein FA, El-Yazbi A, et al. The march of pluripotent stem cells in cardiovascular regenerative medicine. Stem Cell Res Ther 2018; 9(1): 201.
[http://dx.doi.org/10.1186/s13287-018-0947-5] [PMID: 30053890]
[21]
Galach M, Utikal J. From skin to the treatment of diseases--the possibilities of iPS cell research in dermatology. Exp Dermatol 2011; 20(6): 523-8.
[http://dx.doi.org/10.1111/j.1600-0625.2011.01282.x] [PMID: 21585557]
[22]
Larribere L, Utikal J. De- and re-differentiation of the melanocytic lineage. Eur J Cell Biol 2014; 93(1-2): 30-5.
[http://dx.doi.org/10.1016/j.ejcb.2013.11.006] [PMID: 24365127]
[23]
Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger K. A high-efficiency system for the generation and study of human induced pluripotent stem cells. Cell Stem Cell 2008; 3(3): 340-5.
[http://dx.doi.org/10.1016/j.stem.2008.08.003] [PMID: 18786420]
[24]
Saitoh I, Inada E, Iwase Y, et al. Choice of feeders is important when first establishing iPSCs derived from primarily cultured human deciduous tooth dental pulp cells. Cell Med 2015; 8(1-2): 9-23.
[http://dx.doi.org/10.3727/215517915X689038] [PMID: 26858904]
[25]
Eminli S, Utikal J, Arnold K, Jaenisch R, Hochedlinger K. Reprogramming of neural progenitor cells into induced pluripotent stem cells in the absence of exogenous Sox2 expression. Stem Cells 2008; 26(10): 2467-74.
[http://dx.doi.org/10.1634/stemcells.2008-0317] [PMID: 18635867]
[26]
Kar S, Parbin S, Deb M, et al. Epigenetic choreography of stem cells: the DNA demethylation episode of development. Cell Mol Life Sci 2014; 71(6): 1017-32.
[http://dx.doi.org/10.1007/s00018-013-1482-2] [PMID: 24114325]
[27]
Han JW, Yoon YS. Epigenetic landscape of pluripotent stem cells. Antioxid Redox Signal 2012; 17(2): 205-23.
[http://dx.doi.org/10.1089/ars.2011.4375] [PMID: 22044221]
[28]
Takamiya M, Xu F, Suhonen H, et al. Melanosomes in pigmented epithelia maintain eye lens transparency during zebrafish embryonic development. Sci Rep 2016; 6: 25046.
[http://dx.doi.org/10.1038/srep25046] [PMID: 27141993]

Rights & Permissions Print Cite
Article Metrics
14
© 2024 Bentham Science Publishers | Privacy Policy