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Current Pharmaceutical Analysis

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ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

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Research Article

Simultaneous Determination of Four Bioactive Flavonoids in Rat Plasma by UPLC-MS/MS and Comparative Pharmacokinetic Study after Oral Administration of Danyikangtai Powder and Three Compatibilities

Author(s): Yihe Huang, Yanhui Zhao, Yumeng Zhang, Lin Sun, Chunjie Zhao, Xiangdong Zhang and Min Zhao*

Volume 17, Issue 5, 2021

Published on: 30 January, 2020

Page: [629 - 639] Pages: 11

DOI: 10.2174/1573412916666200130112247

Price: $65

Abstract

Background: Danyikangtai powder, a Traditional Chinese Medicine (TCM) formula, shows promise to become a novel drug candidate for the simultaneous treatment of chronic cholecystitis and chronic pancreatitis. However, the pharmacokinetic behavior of Danyikangtai powder remains unclear.

Objective: We investigated the comparative pharmacokinetics of four flavonoids in rats after oral administration of Danyikangtai powder and three compatibilities.

Materials and Methods: The comparative pharmacokinetics was studied by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS). Chromatographic separation was performed on a Universil XB-C18 column with a gradient mobile phase containing 0.1% (v/v) aqueous formic acid and acetonitrile. All analytes and internal standard were quantitated in the multiple reaction monitoring modes with a positive electrospray ionization interface.

Results and Discussion: Danyikangtai powder and Scutellariae radix have similar pharmacokinetic behaviors in rats after oral administration. However, the elimination of four flavonoids in rats after oral administration of Danyikangtai powder was accelerated, which was possibly related to the reduction of the potential hepatotoxicity of Scutellariae radix. The varying degrees of change in pharmacokinetic parameters after oral administration of different herb combinations suggested that herb–herb interactions occurred in vivo.

Conclusion: This study will be helpful to reveal the safety, rational and mechanism of Danyikangtai powder formula compatibility, thereby providing pre-clinical research data for its new drug development and guidance for its rational clinical application.

Keywords: Cholecystitis, pancreatitis, danyikangtai powder, traditional chinese medicine, comparative pharmacokinetics, UPLC–MS/MS.

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[1]
Li, T.; Feng, Z.; Yao, M.; Liao, Q.; Zhao, Z.; Zhang, L. Comparative pharmacokinetic and tissue distribution study of baicalin, baicalein, wogonoside, wogonin and oroxylin-A after oral administration of Component compatibility of SHT and total flavonoids fractions of Radix scutellariae to rats. Anal. Methods, 2014, 6, 5799-5807.
[http://dx.doi.org/10.1039/C4AY00701H]
[2]
Bai, R.C.; Liu, M.; Zhang, X.Q.; Chen, Y.F.; Wang, M.; Zhao, C.J. Simultaneous determination of paeoniflorin, azadirachtin, baicalin and saikosaponin D in Danyikangtai powder by HPLC. J. Shenyang Pharm. Univ., 2017, 34(12), 1072-1076.
[3]
Fong, S.Y.K.; Wong, Y.C.; Zuo, Z. Development of a SPE-LC/MS/MS method for simultaneous quantification of baicalein, wogonin, oroxylin A and their glucuronides baicalin, wogonoside and oroxyloside in rats and its application to brain uptake and plasma pharmacokinetic studies. J. Pharm. Biomed. Anal., 2014, 97, 9-23.
[http://dx.doi.org/10.1016/j.jpba.2014.03.033] [PMID: 24803030]
[4]
Liu, R.X.; Song, G.H.; Wu, P.G.; Zhang, X.W.; Hu, H.J.; Liu, J.; Miao, X.S.; Hou, Z.Y.; Wang, W.Q.; Wei, S.L. Distribution patterns of the contents of five biologically activate ingredients in the root of Scutellaria baicalensis. Chin. J. Nat. Med., 2017, 15(2), 152-160.
[http://dx.doi.org/10.1016/S1875-5364(17)30030-4] [PMID: 28284428]
[5]
Gu, Y.; Chen, X.; Wang, R.; Wang, S.; Wang, X.; Zheng, L.; Zhang, B.; Chai, Y.; Zhu, Z.; Yuan, Y. Comparative two-dimensional HepG2 and L02/cell membrane chromatography/C18/time-of-flight mass spectrometry for screening selective anti-hepatoma components from Scutellariae Radix. J. Pharm. Biomed. Anal., 2019, 164, 550-556.
[http://dx.doi.org/10.1016/j.jpba.2018.10.028] [PMID: 30458388]
[6]
Zhang, Y.; Wang, H.; Liu, Y.; Wang, C.; Wang, J.; Long, C.; Guo, W.; Sun, X. Baicalein inhibits growth of Epstein-Barr virus-positive nasopharyngeal carcinoma by repressing the activity of EBNA1 Q-promoter. Biomed. Pharmacother., 2018, 102, 1003-1014.
[http://dx.doi.org/10.1016/j.biopha.2018.03.114] [PMID: 29710517]
[7]
Xiong, Z.; Jiang, B.; Wu, P.F.; Tian, J.; Shi, L.L.; Gu, J.; Hu, Z.L.; Fu, H.; Wang, F.; Chen, J.G. Antidepressant effects of a plant-derived flavonoid baicalein involving extracellular signal-regulated kinases cascade. Biol. Pharm. Bull., 2011, 34(2), 253-259.
[http://dx.doi.org/10.1248/bpb.34.253] [PMID: 21415537]
[8]
Dinda, B.; Dinda, S.; DasSharma, S.; Banik, R.; Chakraborty, A.; Dinda, M. Therapeutic potentials of baicalin and its aglycone, baicalein against inflammatory disorders. Eur. J. Med. Chem., 2017, 131, 68-80.
[http://dx.doi.org/10.1016/j.ejmech.2017.03.004] [PMID: 28288320]
[9]
Zhang, Y.; Liao, P.; Zhu, M.; Li, W.; Hu, D.; Guan, S.; Chen, L. Baicalin attenuates cardiac dysfunction and myocardial remodeling in a chronic pressure-overload mice model. Cell. Physiol. Biochem., 2017, 41(3), 849-864.
[http://dx.doi.org/10.1159/000459708] [PMID: 28214892]
[10]
Cui, X.B.; Qian, X.C.; Huang, P.; Zhang, Y.X.; Li, J.S.; Yang, G.M.; Cai, B.C. Simultaneous determination of ten flavonoids of crude and wine-processed Radix Scutellariae aqueous extracts in rat plasma by UPLC-ESI-MS/MS and its application to a comparative pharmacokinetic study. Biomed. Chromatogr., 2015, 29(7), 1112-1123.
[http://dx.doi.org/10.1002/bmc.3398] [PMID: 25545174]
[11]
Zhu, Z.; Zhao, L.; Liu, X.; Chen, J.; Zhang, H.; Zhang, G.; Chai, Y. Comparative pharmacokinetics of baicalin and wogonoside by liquid chromatography-mass spectrometry after oral administration of Xiaochaihu Tang and Radix scutellariae extract to rats. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2010, 878(24), 2184-2190.
[http://dx.doi.org/10.1016/j.jchromb.2010.06.021] [PMID: 20643590]
[12]
Di, B.; Feng, N.; Liu, W. Pharmacokinetic comparisons of Shuang-Huang-Lian with the different combinations of its constitutional herbs. J. Ethnopharmacol., 2006, 107(3), 401-405.
[http://dx.doi.org/10.1016/j.jep.2006.03.030] [PMID: 16678990]
[13]
Feng, J.; Xu, W.; Tao, X.; Wei, H.; Cai, F.; Jiang, B.; Chen, W. Simultaneous determination of baicalin, baicalein, wogonin, berberine, palmatine and jatrorrhizine in rat plasma by liquid chromatography-tandem mass spectrometry and application in pharmacokinetic studies after oral administration of traditional Chinese medicinal preparations containing scutellaria-coptis herb couple. J. Pharm. Biomed. Anal., 2010, 53(3), 591-598.
[http://dx.doi.org/10.1016/j.jpba.2010.04.002] [PMID: 20430560]
[14]
Bo, Y.; Wang, L.; Wu, X.; Zhao, L.; Yang, J.; Xiong, Z.; Wu, C. Development and validation of a UHPLC-MS/MS method for the simultaneous determination of five bioactive flavonoids in rat plasma and comparative pharmacokinetic study after oral administration of Xiaochaihu Tang and three compatibilities. J. Sep. Sci., 2017, 40(9), 1896-1905.
[http://dx.doi.org/10.1002/jssc.201601420] [PMID: 28306207]
[15]
Tong, L.; Wan, M.; Zhang, L.; Zhu, Y.; Sun, H.; Bi, K. Simultaneous determination of baicalin, wogonoside, baicalein, wogonin, oroxylin A and chrysin of Radix scutellariae extract in rat plasma by liquid chromatography tandem mass spectrometry. J. Pharm. Biomed. Anal., 2012, 70, 6-12.
[http://dx.doi.org/10.1016/j.jpba.2012.03.051] [PMID: 22703838]
[16]
Li, T.; Wang, Y.W.; Wang, Y.L.; Liang, R.X.; Zhang, D.; Zhang, H.H.; Chen, L.; Zhou, Z.M.; Yang, W.P. LC-MS quantification and pharmacokinetics of the multi-constituents of Huangqin Tang in rat plasma after different single oral doses. Yao Xue Xue Bao, 2013, 48(6), 917-924.
[PMID: 23984529]
[17]
Wang, Y.; Yang, J.; Li, X.; Wang, J. The metabolism of baicalin in rat and the biological activities of the metabolites. Evid. Based Complement. Alternat. Med., 2012, 2012404529
[http://dx.doi.org/10.1155/2012/404529] [PMID: 22654953]
[18]
Taiming, L.; Xuehua, J. Investigation of the absorption mechanisms of baicalin and baicalein in rats. J. Pharm. Sci., 2006, 95(6), 1326-1333.
[http://dx.doi.org/10.1002/jps.20593] [PMID: 16628739]
[19]
Zhang, L.; Lin, G.; Chang, Q.; Zuo, Z. Role of intestinal first-pass metabolism of baicalein in its absorption process. Pharm. Res., 2005, 22(7), 1050-1058.
[http://dx.doi.org/10.1007/s11095-005-5303-7] [PMID: 16028005]
[20]
Xing, J.; Chen, X.; Zhong, D. Absorption and enterohepatic circulation of baicalin in rats. Life Sci., 2005, 78(2), 140-146.
[http://dx.doi.org/10.1016/j.lfs.2005.04.072] [PMID: 16107266]
[21]
Akao, T.; Sakashita, Y.; Hanada, M.; Goto, H.; Shimada, Y.; Terasawa, K. Enteric excretion of baicalein, a flavone of Scutellariae radix, via glucuronidation in rat: involvement of multidrug resistance-associated protein 2. Pharm. Res., 2004, 21(11), 2120-2126.
[http://dx.doi.org/10.1023/B:PHAM.0000048205.02478.b5] [PMID: 15587936]
[22]
Lai, M.Y.; Hsiu, S.L.; Chen, C.C.; Hou, Y.C.; Chao, P.D. Urinary pharmacokinetics of baicalein, wogonin and their glycosides after oral administration of Scutellariae radix in humans. Biol. Pharm. Bull., 2003, 26(1), 79-83.
[http://dx.doi.org/10.1248/bpb.26.79] [PMID: 12520178]
[23]
Zhang, J.; Wang, S.; Xu, L.; Zhang, Q.; Shang, Z.; Zhang, Y.; Wu, Q.; Li, S.; Qiao, Y. Multiple perspectives of qingkailing injection-fraction-single compound in revealing the hepatotoxicity of baicalin and hyodeoxycholic acid. J. Ethnopharmacol., 2018, 215, 147-155.
[http://dx.doi.org/10.1016/j.jep.2017.11.035] [PMID: 29222033]
[24]
Elferink, R.O. Yin Zhi Huang and other plant-derived preparations: Where herbal and molecular medicine meet. J. Hepatol., 2004, 41(4), 691-693.
[http://dx.doi.org/10.1016/j.jhep.2004.08.001] [PMID: 15464257]
[25]
Cernáková, M.; Kostálová, D. Antimicrobial activity of berberine--a constituent of Mahonia aquifolium. Folia Microbiol. (Praha), 2002, 47(4), 375-378.
[http://dx.doi.org/10.1007/BF02818693] [PMID: 12422513]
[26]
Wang, J.R.; Tanaka, T.; Zhang, H.; Kouno, I.; Jiang, Z.H. Formation and conformation of baicalin-berberine and wogonoside-berberine complexes. Chem. Pharm. Bull. (Tokyo), 2012, 60(6), 706-711.
[http://dx.doi.org/10.1248/cpb.60.706] [PMID: 22689420]

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