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Research Article

月桂酸对IPEC-J2细胞分化，增殖和死亡的影响

卷 20, 期 7, 2020

页: [572 - 581] 页: 10

弟呕挨: 10.2174/1566524020666200128155115

价格: $65

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摘要

背景：月桂酸（LA）具有抗菌作用，并有可能替代饲料中的抗生素以防止断奶后腹泻并提高整体猪的生产率。月桂酸对肠上皮细胞的作用尚不清楚。 方法和结果：本研究调查了LA对猪肠道上皮细胞系（IPEC-J2）分化，增殖和死亡的影响，并探讨了其潜在机制。发现0.25-0.1mM LA促进了IPEC-J2细胞分化。在1 mM或更高的浓度下，它诱导IPEC-J2细胞活力降低，脂质蓄积，细胞增殖抑制和细胞凋亡。诱导的细胞死亡不依赖于半胱天冬酶途径。数据表明，LA诱导IPEC-J2细胞自噬，自噬通量受损，自噬在防止LA诱导的细胞死亡中发挥作用。 p38 MAPK抑制剂SB202190减弱了LA降低的IPEC-J2细胞活力。这与自噬水平的增加以及脂质积累和FABPI水平的降低有关。 结论：总之，LA促进IPEC-J2细胞凋亡取决于p38 MAPK途径，并可能涉及自噬和TG代谢调节。

关键词: 月桂酸，IPEC-J2细胞，p38 MAPK，甘油三酸酯，FABPI，自噬。

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