The In Vitro Study of Anti-leishmanial Effect of Naja naja oxiana Snake Venom on Leishmania major

Title:The In Vitro Study of Anti-leishmanial Effect of Naja naja oxiana Snake Venom on Leishmania major

Volume: 20 Issue: 6

Author(s): Nastaran Fallahi, Delavar Shahbazzadeh, Fatemeh Maleki, Marjaneh Aghdasi, Fatemeh Tabatabaie*Khadijeh Khanaliha*

Affiliation:

• Department of Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences, Tehran,Iran

• Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran,Iran

Keywords: Iranian cobra, Naja naja oxiana, Leishmania major, MTT, apoptosis, IC₅₀.

Abstract:

Background: Although a majority of patients with cutaneous leishmaniasis (CL) are healed with Glucantime chemotherapy, the increased drug resistance rate following its application is a concern. In this study, Naja naja oxiana crude venom of cobra snakes was used for the first time as an assembled combination of bioactive pharmaceutical components on Leishmania major (L. major) standard strain.

Objective: To evaluate the efficacy of the Naja naja oxiana crude venom of Iranian cobra snakes on Leishmania major standard strain in vitro.

Methods: Five concentrations (1.25, 2.5, 5, 10 and 20 μg/mL) of venom were added to Leishmania major cultures at 24, 48 and 72 h. The viability of the parasites and venom toxicity were assessed by MTT test. The apoptosis was determined by flow cytometry, while IC₅₀ was determined by counting parasites compared to that of glucantime. Each test was conducted in triplicate.

Results: After the exposure of the venom for 72 h, IC₅₀ value was 0.36 μg/mL and 14.12 μg/mL for promastigotes and amastigotes, respectively. MTT valuation clarified 1.01% promastigotes viability. Furthermore, the results indicated that the venom of Naja naja oxiana induced early and late apoptosis in parasites.

Conclusions: The venom of Naja naja oxiana revealed remarkable anti-leishmanial effects as a novel anti-parasitic alternative. Thus, the bioactive and effective fractions of this venom may be considered as anti-leishmanial candidates in future studies.

Cite this article as:

Fallahi Nastaran , Shahbazzadeh Delavar , Maleki Fatemeh , Aghdasi Marjaneh , Tabatabaie Fatemeh *, Khanaliha Khadijeh *, The In Vitro Study of Anti-leishmanial Effect of Naja naja oxiana Snake Venom on Leishmania major, Infectious Disorders - Drug Targets 2020; 20 (6) . https://dx.doi.org/10.2174/1871526520666200106121839