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当代阿耳茨海默病研究

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ISSN (Print): 1567-2050
ISSN (Online): 1875-5828

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Research Article

阿尔茨海默氏病患者外膜囊泡中的代谢变化：基于LC-MS / MS的代谢组学分析

卷 16, 期 13, 2019

页: [1183 - 1195] 页: 13

弟呕挨: 10.2174/1567205016666191121141352

价格: $65

摘要

目的：表征阿尔茨海默氏病（AD）患者外膜囊泡（OMV）中的特定代谢组学特征，并探讨潜在的代谢生物标志物及其诊断作用。 方法：招募9名AD患者以及年龄和性别相匹配的健康对照者，并收集粪便。提取，纯化OMV，然后使用液相色谱-串联质谱（LC-MS / MS）方法结合一系列多元统计分析进行分析。 结果：AD患者的OMV与健康对照的OMV之间存在显着差异。鉴定了许多不同的代谢产物和几个最重要的代谢途径。证实AD-OMVs中天冬氨酸，L-天冬氨酸，4-唑咪唑和L-谷氨酸的水平高度上调。还鉴定了其他差异代谢物，如花生酸，前列腺素G2和白三烯B4。此外，差异代谢物在ROC曲线（AUC）下具有较高的面积。 结论：AD患者OMVs的代谢活性明显改变。这些数据可能有助于识别新型生物标志物及其在AD中的诊断作用。 此外，OMV代谢组学分析与GWAS结合可以丰富我们对AD遗传谱的了解，并导致更好的AD治疗的早期预测和诊断以及临床应用

关键词: 阿尔茨海默氏病，外膜囊泡，代谢组学，痴呆，LC-MS / MS，全基因组关联研究。

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