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Research Article

miR-7通过视网膜色素上皮细胞中的HoxB3和PI3K / AKT / mTOR信号通路减少高糖诱导的损伤

卷 20, 期 5, 2020

页: [372 - 378] 页: 7

弟呕挨: 10.2174/1566524019666191023151137

价格: $65

摘要

背景：糖尿病性视网膜病（DR）是糖尿病的常见并发症。这项研究调查了miR-7通过HoxB3基因和DR中的PI3K / AKT / mTOR信号通路调节细胞增殖的作用。 方法：在正常培养基（对照）和高葡萄糖培养基（25mM葡萄糖，HG）中培养的人视网膜色素上皮细胞系（ARPE-19）分别用模拟NC（HG +模拟NC），miR-7模拟（HG + miR-）转染。 7个模拟物），抑制剂NC（HG +抑制剂NC）和miR抑制剂（HG + miR-7抑制剂）。测定细胞的存活力，凋亡和基因表达。 结果：HG降低细胞活力并增加凋亡。但是，miR-7模拟物减少了细胞凋亡。 PCR结果显示，用miR-7模拟物转染后，miR-7明显上调。在miR-7模拟转染后，Hoxb3，mTOR，p-PI3K和p-AKT的表达在mRNA和蛋白质水平上显着下调，而PI3K和AKT的表达没有差异。 结论：我们的研究结果表明，miR-7通过各种途径调节视网膜上皮细胞的生长，并且是预防和治疗糖尿病性视网膜病的潜在治疗靶标。

关键词: miR-7，HoxB3，DR，PI3K / AKT / mTOR信号传导，视网膜病变，细胞增殖。

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