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Letter Article

慢性阻塞性肺疾病和肺癌的高通量多重SNP分析

卷 20, 期 3, 2020

页: [185 - 193] 页: 9

弟呕挨: 10.2174/1566524019666191017123446

价格: $65

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摘要

背景：已经显示出许多人类炎症性疾病和肿瘤以特定方式引起血浆蛋白糖基化模式的改变。这些高度可变和通用的翻译后修饰通过影响分选，折叠，酶活性和亚细胞定位来微调蛋白质功能。然而，关于该过程的调节因素以及糖基化与疾病之间的确切因果关系的了解相对较少。 目的：本研究的目的是研究编码糖基转移酶和糖苷酶的基因中的某些单核苷酸多态性（SNP）是否可能与慢性阻塞性肺疾病（COPD）和肺腺癌的高风险相关。 方法：选择位于与N-糖基化相关的基因中的32个SNP进行关联分析。募集具有假定的生物学功能（错义或调控变体）的多态性。通过TaqMan OpenArray平台对SNP进行基因分型。基于单碱基扩展的方法与毛细管凝胶电泳相结合用于验证。 结果：TaqMan OpenArray方法提供了准确可靠的基因型数据（全局调用率：94.9％，准确性：99.6％）。如果任何SNP证实了可靠的采样和基因分型，则在健康对照组的样本组中，在获得的基因型频率值和预期的基因型频率值（Hardy-Weinberg平衡）之间未检测到显着差异。在所比较的样本组之间，位于MGAT5基因3''UTR的rs3944508多态性的等位基因频率显着不同。 结论：我们的结果表明rs34944508 SNP可能通过影响MGAT5的表达来调节肺癌的风险。此酶催化在双天线N连接寡糖的α连接甘露糖上以β1-6连接形式添加N-乙酰氨基葡萄糖（GlcNAc），因此增加了侵入性恶性肿瘤的特征性分支。

关键词: SNP，单碱基引物延伸，毛细管电泳，TaqMan OpenArray，肺腺癌，COPD，遗传关联。

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