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当代肿瘤药物靶点

Editor-in-Chief

ISSN (Print): 1568-0096
ISSN (Online): 1873-5576

Research Article

ERGIC3沉默可增加BFA对肺腺癌细胞的生长抑制作用

卷 20, 期 1, 2020

页: [67 - 75] 页: 9

弟呕挨: 10.2174/1568009619666190917145906

价格: $65

摘要

背景:已知布雷菲德菌素A(BFA)在癌细胞中诱导内质网应激(ERS)和高尔基体应激。 ERGIC3(内质网-高尔基体中间隔室3)是位于内质网和高尔基体中的II型跨膜蛋白。在癌细胞中经常观察到ERGIC3过表达。目的:在这项研究中,我们旨在探讨BFA与ERGIC3沉默同时给药是否会相加或协同抑制癌细胞的生长。 方法:用ERGIC3-siRNA敲低ERGIC3的表达,用BFA诱导肺癌细胞GLC-82和A549中的ERS。使用Q-RT-PCR和Western Blot分析检测ERGIC3和下游分子的表达。 GraphPad Prism 6用于量化数据。 结果:我们证明了通过siRNA沉默ERGIC3可以有效降低GLC-82和A549细胞中mRNA和蛋白质水平上ERGIC3的表达。虽然单独使用BFA或ERGIC3-沉默可以诱导ERS并抑制细胞生长,但是将ERGIC3-silencing和BFA联合用于肺癌细胞治疗可以通过上调GRP78来加重增强细胞生长的抑制作用,从而导致细胞周期停滞。 结论:ERGIC3沉默联合BFA治疗可加成抑制肺癌细胞的生长。这一发现可能为肺腺癌的新辅助疗法提供了启示。

关键词: ERGIC3,BFA,肺癌,细胞生长,内质网应激,腺癌细胞。

图形摘要

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