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Current Pharmaceutical Analysis

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ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

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Review Article

Metabolite Detection and Profiling Using Analytical Methods

Author(s): Lovekesh Mehta*, Parul Grover, Tanveer Naved and Debaraj Mukherjee

Volume 17, Issue 1, 2021

Published on: 06 September, 2019

Page: [2 - 9] Pages: 8

DOI: 10.2174/1573412915666190906142536

Price: $65

Abstract

To develop effective and safe drugs and to take them to the market in short period of time is the mission of pharmaceutical research companies. A selection of few of the lead compounds are done for the evaluation of safety and their ADMET (absorption, distribution, metabolism, excretion and toxicology) properties are tested in in-vitro (test systems), in-vivo (living organisms) and in-silico (computational methods). From initial stages to final stages of modern drug discovery processes, the vital tool for detecting and characterizing metabolites is MS (Mass spectrometry) hyphenated with other techniques. The methods used for generation of metabolites are in vitro techniques and cell lines (containing expressing drug metabolizing enzymes and heterologous genes). The use of HPLC-MS/UPLC-MS and high resolution MS, enables the in depth metabolite detection and profiling studies and it may also be likely to identify and characterize the site and types of biotransformation.

Keywords: Forced degradation study, biotransformation, regioselective, preformulation, ICH, pharmacokinetics.

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Graphical Abstract

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