Login Register Cart 0
Generic placeholder image

Current Chemical Biology

Editor-in-Chief

ISSN (Print): 2212-7968
ISSN (Online): 1872-3136

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Specific Enzymatic Activity of Ceruloplasmin as a Potential Indicator of Copper Status

Author(s): Kuldip Upadhyay* and Ankit Viramgami

Volume 13, Issue 3, 2019

Page: [250 - 256] Pages: 7

DOI: 10.2174/2212796813666190903115853

Abstract

Background: The serum copper (Cu) and ceruloplasmin (Cp) concentrations are common blood markers of copper metabolism. In altered physiological conditions, Cp can act as an acute phase reactant and its concentration may increase.

Objective: To evaluate specific enzymatic activity of Cp as a potential indicator of Cu status and its correlation with serum Cu level.

Methods: Serum Cu levels were estimated as per NIOSH method. Specific enzymatic activity of Cp was determined from enzymatic activity and immune concentration of Cp as per standard methods. The statistical analysis was carried out using the package of social science (SPSS) software.

Results: The difference in mean specific enzymatic activity of Cp was statistically significant between clinical and control groups. In control population, the correlation between serum Cu level and specific enzymatic activity of Cp was moderate and statistically significant (r=0.566, p=0.014, N=18) as compared to the clinical group (r=0.338, p=0.016, N=50).

Conclusion: The study revealed that clinical group was significantly different in specific enzymatic activity of Cp as compared to control group. Besides this, the specific enzymatic activity of Cp was moderately but significantly correlated with serum Cu level in control group but did not reveal conclusive evidence in clinical population.

Keywords: Serum Cu level, ceruloplasmin, enzymatic activity of Cp, immune concentration of Cp, specific enzymatic activity of Cp, acute phase reactant.

« Previous Next »
Graphical Abstract

[1]
Organization WH. IPCS environmental health criteria 200 Copper. Vammala, Finland: World Health Organization 1998.
[2]
Uriu-Adams JY, Keen CL. Copper, oxidative stress, and human health. Mol Aspects Med 2005; 26(4-5): 268-98.
[http://dx.doi.org/10.1016/j.mam.2005.07.015] [PMID: 16112185]
[3]
Stark AA, Glass GA. Role of copper and ceruloplasmin in oxidative mutagenesis induced by the glutathione-gamma-glutamyl transpeptidase system and by other thiols. Environ Mol Mutagen 1997; 29(1): 63-72.
[http://dx.doi.org/10.1002/(SICI)1098-2280(1997)29:1<63:AID-EM9>3.0.CO;2-E] [PMID: 9020309]
[4]
Twomey PJ, Viljoen A, House IM, Reynolds TM, Wierzbicki AS. Relationship between serum copper, ceruloplasmin, and non-ceruloplasmin-bound copper in routine clinical practice. Clin Chem 2005; 51(8): 1558-9.
[http://dx.doi.org/10.1373/clinchem.2005.052688] [PMID: 16040861]
[5]
Araya M, Kelleher SL, Arredondo MA, et al. Effects of chronic copper exposure during early life in rhesus monkeys. Am J Clin Nutr 2005; 81(5): 1065-71.
[http://dx.doi.org/10.1093/ajcn/81.5.1065] [PMID: 15883430]
[6]
Harvey LJ, Majsak-Newman G, Dainty JR, et al. Adaptive responses in men fed low- and high-copper diets. Br J Nutr 2003; 90(1): 161-8.
[http://dx.doi.org/10.1079/BJN2003887] [PMID: 12844388]
[7]
Turnlund JR, Keyes WR, Anderson HL, Acord LL. Copper absorption and retention in young men at three levels of dietary copper by use of the stable isotope 65Cu. Am J Clin Nutr 1989; 49(5): 870-8.
[http://dx.doi.org/10.1093/ajcn/49.5.870] [PMID: 2718922]
[8]
Turnlund JR, Keyes WR, Peiffer GL, Scott KC. Copper absorption, excretion, and retention by young men consuming low dietary copper determined by using the stable isotope 65Cu. Am J Clin Nutr 1998; 67(6): 1219-25.
[http://dx.doi.org/10.1093/ajcn/67.6.1219] [PMID: 9625096]
[9]
Danzeisen R, Araya M, Harrison B, et al. How reliable and robust are current biomarkers for copper status? Br J Nutr 2007; 98(4): 676-83.
[http://dx.doi.org/10.1017/S0007114507798951] [PMID: 17666147]
[10]
Olivares M, Méndez MA, Astudillo PA, Pizarro F. Present situation of biomarkers for copper status. Am J Clin Nutr 2008; 88(3): 859S-62S.
[http://dx.doi.org/10.1093/ajcn/88.3.859S] [PMID: 18779309]
[11]
Rinaldi AC. Meeting report–Copper research at the top. Biometals an international journal on the role of metal ions in biology, biochemistry, and medicine 2000; 13(1): 9-13.
[12]
Araya M, Olivares M, Pizarro F, Gonzalez M, Speisky H, Uauy R. Copper exposure and potential biomarkers of copper metabolism.Biometals : an international journal on the role of metal ions in biology,biochemistry, and medicine 2003; 16(1): 199-204.
[13]
Hambidge M. Biomarkers of trace mineral intake and status. J Nutr 2003; 133(3): 948S-55S.
[http://dx.doi.org/10.1093/jn/133.3.948S] [PMID: 12612181]
[14]
Barber EF, Cousins RJ. Interleukin-1--stimulated induction of ceruloplasmin synthesis in normal and copper-deficient rats. J Nutr 1988; 118(3): 375-81.
[http://dx.doi.org/10.1093/jn/118.3.375] [PMID: 3258371]
[15]
Denko CW. Protective role of ceruloplasmin in inflammation. Agents Actions 1979; 9(4): 333-6.
[http://dx.doi.org/10.1007/BF01970657] [PMID: 517330]
[16]
Danks DM, Campbell PE, Walker-Smith J, et al. Menkes’ kinky-hair syndrome. Lancet 1972; 1(7760): 1100-2.
[http://dx.doi.org/10.1016/S0140-6736(72)91433-X] [PMID: 4112576]
[17]
Gibbs K, Walshe JM. A study of the caeruloplasmin concentrations found in 75 patients with Wilson’s disease, their kinships and various control groups. Q J Med 1979; 48(191): 447-63.
[PMID: 396544]
[18]
Spechler SJ, Koff RS. Wilson’s disease: diagnostic difficulties in the patient with chronic hepatitis and hypoceruloplasminemia. Gastroenterology 1980; 78(4): 803-6.
[http://dx.doi.org/10.1016/0016-5085(80)90687-3] [PMID: 7353765]
[19]
Milne DB, Johnson PE, Klevay LM, Sandstead HH. Effect of copper intake on balance, absorption, and status indices of copper in men. Nutr Res 1990; 10(9): 975-86.
[http://dx.doi.org/10.1016/S0271-5317(05)80039-9]
[20]
Milne DB, Klevay LM, Hunt JR. Effects of ascorbic acid supplements and a diet marginal in copper on indices of copper nutriture in women. Nutr Res 1988; 8(8): 865-73.
[http://dx.doi.org/10.1016/S0271-5317(88)80126-X]
[21]
Ritland S, Skrede S, Johansen O. A long-term follow-up study of the hepatic copper and serum ceruloplasmin concentrations in patients with chronic liver disease. Scand J Gastroenterol 1982; 17(4): 545-51.
[http://dx.doi.org/10.3109/00365528209182247] [PMID: 7134882]
[22]
Milne DB. Assessment of copper nutritional status. Clin Chem 1994; 40(8): 1479-84.
[PMID: 8044985]
[23]
Milne DB, Johnson PE. Assessment of copper status: effect of age and gender on reference ranges in healthy adults. Clin Chem 1993; 39(5): 883-7.
[PMID: 8387409]
[24]
Eller PM, Cassinelli ME. NIOSH manual of analytical methods. Diane Publishing 1994.
[25]
Schosinsky KH, Lehmann HP, Beeler MF. Measurement of ceruloplasmin from its oxidase activity in serum by use of o-dianisidine dihydrochloride. Clin Chem 1974; 20(12): 1556-63.
[PMID: 4214636]
[26]
Kratz A, Ferraro M, Sluss PM, Lewandrowski KB. Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Laboratory reference values. N Engl J Med 2004; 351(15): 1548-63.
[http://dx.doi.org/10.1056/NEJMcpc049016] [PMID: 15470219]

Rights & Permissions Print Cite
Article Metrics
13
2
© 2024 Bentham Science Publishers | Privacy Policy