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The Natural Products Journal

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ISSN (Print): 2210-3155
ISSN (Online): 2210-3163

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Research Article

Gastroprotective Effect of Chinese Cabbage (Brassica oleracea L. var. pekinensis) Juice in Sprague Dawley Rats

Author(s): Jin Han Chin*, Kah Hui Wong and Soh Onn Yeong

Volume 10, Issue 5, 2020

Page: [587 - 594] Pages: 8

DOI: 10.2174/2210315509666190902111029

Price: $65

Abstract

Background: Chinese cabbage or Brassica oleracea L. var. pekinensis is an edible leafy green vegetable in the family of Brassicaceae. Ethnopharmacological studies have shown that the juice of cabbage leaves is commonly used in the treatment of gastrointestinal disorders, irritable bowel syndrome, minor cuts and wounds and mastitis.

Objective: This study was aimed to examine the gastroprotective effect of Chinese cabbage (Family: Brassicaceae) juice in rats by using ethanol-induced gastric ulcer model.

Methods: Thirty albino male Sprague Dawley rats (150 ± 20 g) were divided into 6 groups with five rats (n = 5) in each group. The normal control group was treated with distilled water while the positive control group was intragastrically administered with omeprazole (20 mg/kg). Cabbage juice at 500 mg/kg was given orally to three experimental groups for 1-day, 7-day and 14-day, respectively. Ethanol (70 % v/v) was orally treated to all rats one hour after the last dose treatment except the normal control group.

Results: Results obtained showed that repeated consumption of cabbage juice for 7 days and 14 days exhibited an increase in accumulation mucus and in the levels of superoxide dismutase (SOD), glutathione- s-transferase (GST) and glutathione peroxidase (GSH-Px) as well as a reduction in gastric lesion area induced by ethanol compared with the ulceration control group.

Conclusion: In conclusion, the gastroprotective effect of Chinese cabbage juice could be associated with its ability to increase endogenous antioxidant activities of SOD, GST and GSH-Px and mucus secretion in rat stomach.

Keywords: Antioxidant, Chinese cabbage juice, gastric mucosa, glutathione, histology, superoxide dismutase.

Graphical Abstract

[1]
Sung, J.J.Y.; Kuipers, E.J.; El-Serag, H.B. Systematic review: The global incidence and prevalence of peptic ulcer disease. Aliment. Pharmacol. Ther., 2009, 29(9), 938-946.
[http://dx.doi.org/10.1111/j.1365-2036.2009.03960.x ] [PMID: 19220208 ]
[2]
Gomes Silva, M.I.; Florenço de Sousa, F.C. Gastric ulcer etiology. Peptic ulcer disease; Intech Open: London, 2011, pp. 1-28.
[http://dx.doi.org/10.5772/20796]
[3]
Cederbaum, A.I. Introduction-serial review: Alcohol, oxidative stress and cell injury. Free Radic. Biol. Med., 2001, 31(12), 1524-1526.
[http://dx.doi.org/10.1016/S0891-5849(01)00741-9 ] [PMID: 11744324]
[4]
Oguwike, F.N.; Offor, C.C.; Nwadighoha, A.N.; Ebede, E.O. Evaluation of efficacy of cabbage juice (Brassica oleracea Linne) as potential antiulcer agent and its effect on the haemostatic mechanism of male albino wistar rats. IOSR J. Dental Med. Sc., 2014, 13(1), 92-97.
[5]
ˇSamec, D.; Piljac-ˇZegarac, J.; Bogovi c, M.; Habjaniˇc, K.; Grúz, J. Antioxidant potency of white (Brassica oleracea L. var. capitata) and Chinese (Brassica rapa L. var. pekinensis (Lour.) cabbage: The influence of development stage, cultivar choice and seed selection. Sci. Hortic. (Amsterdam), 2011, 128(2), 78-83.
[http://dx.doi.org/10.1016/j.scienta.2011.01.009]
[6]
Brighente, I.M.C.; Dias, M.; Verdi, L.G.; Pizzolatti, M.G. Antioxidant activity and total phenolic content of some Brazilian species. Pharm. Biol., 2007, 45(2), 156-161.
[http://dx.doi.org/10.1080/13880200601113131]
[7]
Nor Qhairul, I.M.; Fadzelly, M. Phytochemicals and antioxidant properties of different parts of Camellia sinesis leaves from Sabah tea plantation in Sabah Malaysia. Int. Food Res. J., 2013, 20(1), 307-312.
[8]
Sharma, O.P.; Bhat, T.K. DPPH antioxidant assay revisited. Food Chem., 2009, 113(4), 1202-1205.
[http://dx.doi.org/10.1016/j.foodchem.2008.08.008]
[9]
Park, C.H.; Nam, D.Y.; Son, H.U.; Lee, S.R.; Lee, H.J.; Heo, J.C.; Cha, T.Y.; Baek, J.H.; Lee, S.H. Polymer fraction of Aloe vera exhibits a protective activity on ethanol-induced gastric lesions. Int. J. Mol. Med., 2011, 27(4), 511-518.
[PMID: 21286662]
[10]
Al Mofleh, I.A. Spices, herbal xenobiotics and the stomach: Friends or foes? World J. Gastroenterol., 2010, 16(22), 2710-2719.
[http://dx.doi.org/10.3748/wjg.v16.i22.2710 ] [PMID: 20533590]
[11]
Mota, K.S.; Dias, G.E.N.; Pinto, M.E.F.; Luiz-Ferreira, A.; Souza-Brito, A.R.M.; Hiruma-Lima, C.A.; Barbosa-Filho, J.M.; Batista, L.M. Flavonoids with gastroprotective activity. Molecules, 2009, 14(3), 979-1012.
[http://dx.doi.org/10.3390/molecules14030979 ] [PMID: 19305355]
[12]
Ichikawa, T.; Ishihara, K. Protective effects of gastric mucus, 2011.
[13]
Nimse, S.B.; Pal, D. Free radicals, natural antioxidants, and their reaction mechanisms. RSC Advances, 2015, 5, 27986-28006.
[http://dx.doi.org/10.1039/C4RA13315C]
[14]
Boyacioglu, M.; Kum, C.; Sekkin, S.; Yalinkilinc, H.S.; Avci, H.; Epikmen, E.T.; Karademir, U. The effects of lycopene on DNA damage and oxidative stress on indomethacin-induced gastric ulcer in rats. Clin. Nutr., 2016, 35(2), 428-435.
[http://dx.doi.org/10.1016/j.clnu.2015.03.006 ] [PMID: 25818123]
[15]
Chen, J.; He, Z.; Liu, H.; Cha, C. Electrochemical determination of reduced gluthation (GSH) by applying the powder microelectrode technique. J. Electroanal. Chem. (Lausanne Switz.), 2006, 588(2), 324-330.
[http://dx.doi.org/10.1016/j.jelechem.2006.01.008]
[16]
Gao, C.M.; Takezaki, T.; Wu, J.Z.; Li, Z.Y.; Liu, Y.T.; Li, S.P.; Ding, J.H.; Su, P.; Hu, X.; Xu, T.L.; Sugimura, H.; Tajima, K. Glutathione-S-transferases M1 (GSTM1) and GSTT1 genotype, smoking, consumption of alcohol and tea and risk of esophageal and stomach cancers: a case-control study of a high-incidence area in Jiangsu Province, China. Cancer Lett., 2002, 188(1-2), 95-102.
[http://dx.doi.org/10.1016/S0304-3835(02)00115-5 ] [PMID: 12406553]
[17]
Repetto, M.G.; Llesuy, S.F. Antioxidant properties of natural compounds used in popular medicine for gastric ulcers. Braz. J. Med. Biol. Res., 2002, 35(5), 523-534.
[http://dx.doi.org/10.1590/S0100-879X2002000500003 ] [PMID: 12011936]

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