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Current Drug Discovery Technologies

Editor-in-Chief

ISSN (Print): 1570-1638
ISSN (Online): 1875-6220

Research Article

Zebra Fish in Toxicology Research: Streptavidin Conjugated Peroxidase Assay in the Development Phase of Zebrafish Embryos to Study Liver Toxicities

Author(s): Asis Bala*, Chaitali Mondal and Pallab KantiHaldar

Volume 17, Issue 5, 2020

Page: [735 - 739] Pages: 5

DOI: 10.2174/1570163816666190801120016

Price: $65

Abstract

Background: Zebrafish have similar hepatic anatomy and cellular architecture just like mammals. Therefore, a number of investigators are using zebrafish to study liver pathologies. However, the evaluation model specific to liver toxicities in zebra fish was not clearly stated earlier. Aims and Objectives: The present study was designed to develop a model of embryonic liver toxicity using dexamethasone (DEXA, 0-20 μM) as a standard hepatotoxic agent.

Methods: such toxicities are easily measured by streptavidin-conjugated peroxidase assay after 48- hour post-fertilization (hpf) of DEXA treatment.

Results: In addition to morphological toxicities at different hpf, DEXA showed significant (*p< 0.05 &**p<0.01) reduction of peroxidase-chromogenic dye reaction in the assay as compared to DEXA untreated embryos at 10 & 20 μM concentration that concluded the hepatocellular toxicity of dexamethasone.

Conclusion: Hopefully, the developed model for hepatotoxicity evaluation will be a promising model for the evaluation of new drugs or chemicals as an easy vertebrate model before the commencement of another animal model.

Keywords: Zebra fish, embryonic liver toxicity, streptavidin conjugated peroxidase assay, toxicology, hepatic anatomy, mammals.

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